NDLI: Spontaneous Calcium Transients in Cultured Cortical Networks During Development

Content Provider	IEEE Xplore Digital Library
Author	Takayama, Y. Moriguchi, H. Kotani, K. Jimbo, Y.
Copyright Year	1964
Abstract	Spontaneous neuronal activity plays an important role in the development of the brain. Developmental changes in the spontaneous activity pattern of neuronal networks in vitro have been extensively studied by using the microelectrode array (MEA) recording system. However, little is known about the transition of spontaneous intracellular calcium dynamics, and the relationship between calcium transients and electrical activity during development. In the present paper, we carry out simultaneous recording of spontaneous electrical activity and intracellular calcium transients of rat cortical networks cultured on MEA. In one-week cultures, periodic synchronized bursts are observed and are followed by synchronized calcium transients. In three-week cultures, synchronized calcium transients are rarely observed despite the presence of highly complicated synchronized activity. Between these two states, in two-week cultures, slow, radial propagation of calcium waves independent of electrical activity is observed. Pharmacological treatments with the purinergic receptor antagonist suramin and gap junction blocker 18-beta glycyrrhetinic acid reveal that the spontaneous radial calcium waves are mediated by the astrocytic network, and suggest that the astrocytic calcium waves can influence the electrical firing patterns of networks by locally affecting neuronal signaling. These results indicate that the various dynamics of intracellular calcium transients regulate the network maturation processes.
Sponsorship	IEEE Engineering in Medicine and Biology Society
Page Count	8
File Size	753486
Starting Page	2949
Ending Page	2956
File Format	PDF
ISSN	00189294
Volume Number	56
Issue Number	12
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2009-12-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Calcium Biological neural networks Spatiotemporal phenomena Microelectrodes Neurons Humans IEEE activities In vitro Nervous system Morphology microelectrode array (MEA) Calcium transients calcium waves cultured cortical networks developmental changes
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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