NDLI: Label-Free DNA Detection Using a Charge Sensitive CMOS Microarray Sensor Chip

Content Provider	IEEE Xplore Digital Library
Author	Musayev, J. Adlguzel, Y. Kulah, H. Eminoglu, S. Akln, T.
Copyright Year	2001
Abstract	This paper presents label-free DNA detection using a charge sensitive microarray sensor. The microarray sensor, fabricated with a standard CMOS process, contains 1024 detector elements integrated together with their readout circuit in a single chip. This microarray sensor chip is developed for biosensing applications involving label-free detection of charged particles and molecules with improved sensitivity. The proposed chip is used for the detection of DNA immobilization and hybridization by directly sensing the phosphate backbone charge of DNA molecules. The sensing part of the chip consists of an array of 7 μm × 7 μm capacitive metal electrodes arranged in 32 × 32 format with a pitch of 15 μm, which allows implementation of a portable and low-cost microarray sensor. The sensitivity of the microarray sensor is improved compared with other direct charge sensing CMOS biosensors, using low-noise detection and amplification circuits, and implementing correlated double sampling, which reduces the input referred rms noise level down to 6.8 electrons (e^-). Due to this low noise level, detection of DNA having 1 pM concentration is achieved, showing that the chip is much more sensitive than its counterparts, and even as sensitive as conventional fluorescence or gravimetric detection techniques. A dynamic range of 70 dB is achieved, along with the low noise level. The tests were performed with 10 base pairs DNA in 13- μM probe (5'-TCTCACCTTC-3') and 1-pM target (3'-AGAGTGGAAG-5') oligomer solutions. In both cases, charges of the DNA molecules interacting with the surface were successfully detected.
Sponsorship	IEEE Sensors Council
Starting Page	1608
Ending Page	1616
Page Count	9
File Size	2314600
File Format	PDF
ISSN	1530437X
Volume Number	14
Issue Number	5
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2014-01-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Detectors DNA Sensitivity Semiconductor device measurement Voltage measurement Noise label-free detection Charge sensor CMOS microarray
Content Type	Text
Resource Type	Article
Subject	Instrumentation Electrical and Electronic Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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