NDLI: Techniques for Obtaining Absorption Spectra on Intact Biological Samples

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Techniques for Obtaining Absorption Spectra on Intact Biological Samples

Content Provider	IEEE Xplore Digital Library
Author	Norris, K. H. Butler, W. L.
Copyright Year	1961
Abstract	Absorption spectra can be obtained on a wide range of biological samples with little or no sample preparation by using a high sensitivity, low-noise spectrophotometer with the sample in close juxtaposition with the photocathode. An instrument designed for such measurements is described, and possible applications are discussed. The spectrophotometer is a single-beam recording unit using a double-prism monochromator, 100-watt tungsten source, end-window multiplier-type phototube and an X-Y recorder. The phototube is operated at a constant anode current and a logarithmic voltmeter measures the dynode voltage, providing a photometer which is linear with density change over an opticaldensity range of 8. The noise level for samples of low density is equivalent to an optical-density change of 0.002 with a response time of 1 second for full scale pen travel. Any part or all of the wavelength region from 200 to 1200 m¿ may be scanned with a wide choice of scanning speeds. Provision is included for electrical correction of system response to give a flat baseline characteristic for a selected region of the spectrum. This permits measurements at high sensitivity on samples with high scatter loss. Versatile sample mounting arrangements permit measurement of a wide range of materials. Liquids, powders, and homogenates are measured in sample cells of appropriate size. Tissue slices and solid samples are mounted on a metal plate with an aperture in the center for the transmitted light to reach the phototube.
Starting Page	153
Ending Page	157
Page Count	5
File Size	952176
File Format	PDF
ISSN	00961884
Volume Number	8
Issue Number	3
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	1961-07-01
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Absorption Optical recording Photoelectricity Optical scattering Biomedical optical imaging Optical noise Optical sensors Optical variables control Cathodes Instruments
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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