NDLI: Directed Metallization of Single-Enzyme Molecules With Preserved Enzymatic Activity

Content Provider	IEEE Xplore Digital Library
Author	Vernick, S. Moscovich-Dagan, H. Porat-Ophir, C. Rishpon, J. Freeman, A. Shacham-Diamand, Y.
Copyright Year	2002
Abstract	A new method for the fabrication of molecular, water-soluble, and biologically active enzyme-metal hybrids was designed and its feasibility demonstrated. The method is based on the display of nucleation sites directing a subsequent electroless deposition of palladium and other metals to the enzyme's surface. The process is carried out under mild physiological conditions, enabling the preservation of enzymatic activity and water solubility. The feasibility of the new method was demonstrated by using the enzyme glucose oxidase and palladium combination as the first model system. The glucose oxidase-palladium hybrid thus obtained retained their solubility and enzymatic glucose oxidation capabilities. Hybrids immobilized on platinum electrodes exhibited ldquonanowiringrdquo and effective direct electron transfer from the enzyme catalytic site to the electrode. The new enzyme-metal hybrids thus obtained may be readily incorporated into miniaturic biosensors and biochips, used as novel antibacterial agents or as markers for improved in vivo imaging. Furthermore, the methodology developed may be readily extended to a series of metal coatings on the surface of biologically active proteins.
Sponsorship	IEEE Nanotechnology Council
Starting Page	95
Ending Page	99
Page Count	5
File Size	335715
File Format	PDF
ISSN	1536125X
Volume Number	8
Issue Number	1
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2009-01-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Metallization Sugar Palladium Biochemistry Electrodes Fabrication Displays Oxidation Platinum Electrons metallization Biosensors electroless deposition metal–enzyme hybrid
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Electrical and Electronic Engineering Computer Science Applications

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
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