NDLI: Oxygen sensor based on the fluorescence quenching of a ruthenium complex immobilized in a biocompatible Poly(Ethylene glycol) hydrogel

Content Provider	IEEE Xplore Digital Library
Author	O'Neal, D.P. Meledeo, M.A. Davis, J.R. Ibey, B.L. Gant, V.A. Pishko, M.V. Cote, G.L.
Copyright Year	2001
Abstract	An optically based system has been developed for use as an oxygen sensor for a cell culture bioreactor. Electrochemical sensors based on the Clark oxygen electrode are typically used with cell-culture bioreactors. These sensors, however, are subject to long-term drift, due in part to biofouling, and require penetrating the bioreactor with the probe in order to perform a measurement. We report an implantable sensor that, when used with an external fiber-optic probe, takes advantage of the oxygen stimulated fluorescence quenching of dichloro(tris-1,10-phenanthroline) ruthenium (II) hydrate. This fluorophore was immobilized in a photopolymerized hydrogel made from poly(ethylene glycol) diacrylate (PEG-DA), a polymer known to minimize protein and cell adhesion. A low-average molecular weight PEG-DA (MW = 575) was employed to hinder the fluorophore from leaching. The PEG-DA precursor solution contained 40% H/sub 2/O such that, upon polymerization, the gel was already in the hydrated state. Sensor hydrogels stored in H/sub 2/O for several months retained their physical shape and sensitivity to oxygen. The sensor showed a high degree of reproducibility across a range of oxygen concentrations that are typical for cell culture experiments (0-9.1 ppm O/sub 2/), and a linear model produced a strong correlation (R /sup 2/= 0.995) compared with a commercial electrochemical probe. No drift or hysteresis was identified in the sensor across cycles of varying oxygen concentrations in this range.
Sponsorship	IEEE Sensors Council
Starting Page	728
Ending Page	734
Page Count	7
File Size	790486
File Format	PDF
ISSN	1530437X
Volume Number	4
Issue Number	6
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2004-12-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Oxygen Biosensors Fluorescence Bioreactors Probes Optical sensors Biomedical optical imaging Sensor systems Electrodes Performance evaluation ruthenium Oxygen fiber-optic sensor poly(ethylene glycol)
Content Type	Text
Resource Type	Article
Subject	Instrumentation Electrical and Electronic Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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