NDLI: Feature representation and signal classification in fluorescence in-situ hybridization image analysis

Content Provider	IEEE Xplore Digital Library
Author	Lerner, B. Clocksin, W.F. Dhanjal, S. Hulten, M.A. Bishop, C.M.
Copyright Year	1996
Abstract	Fast and accurate analysis of fluorescence in-situ hybridization images for signal counting will depend mainly upon two components: a classifier to discriminate between artifacts and valid signals of several fluorophores (colors), and well discriminating features to represent the signals. Our previous work (2001) has focused on the first component. To investigate the second component, we evaluate candidate feature sets by illustrating the probability density functions and scatter plots for the features. The analysis provides first insight into dependencies between features, indicates the relative importance of members of a feature set, and helps in identifying sources of potential classification errors. Class separability yielded by different feature subsets is evaluated using the accuracy of several neural network-based classification strategies, some of them hierarchical, as well as using a feature selection technique making use of a scatter criterion. Although applied to cytogenetics, the paper presents a comprehensive, unifying methodology of qualitative and quantitative evaluation of pattern feature representation essential for accurate image classification. This methodology is applicable to many other real-world pattern recognition problems.
Sponsorship	IEEE Systems, Man, and Cybernetics Society
Starting Page	655
Ending Page	665
Page Count	11
File Size	182827
File Format	PDF
ISSN	10834427
Volume Number	31
Issue Number	6
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2001-11-01
Publisher Place	U.S.A.
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Pattern classification Fluorescence Image color analysis Scattering Image analysis Signal analysis Probability density function Neural networks Image classification Pattern recognition
Content Type	Text
Resource Type	Article
Subject	Control and Systems Engineering Information Systems Electrical and Electronic Engineering Human-Computer Interaction Computer Science Applications Software

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in