NDLI: Simultaneous Holographic Microscopy and Raman Spectroscopy Monitoring of Human Spermatozoa Photodegradation

Content Provider	IEEE Xplore Digital Library
Author	Ferrara, M.A. De Angelis, A. De Luca, A.C. Coppola, G. Dale, B. Coppola, G.
Copyright Year	1995
Abstract	Coupling digital holography with high-specific Raman spectroscopy is an attractive means of identifying biochemical changes in cells by both physical topography and spectroscopy. We have used this approach to simultaneously study biochemical and morphological characteristics of human sperm cells irradiated with green laser radiation. Severe spermatozoa variations associated with a topological redistribution of the sample and a gradual decrease in the Raman signal intensity were detected in a label-free configuration. Importantly, at laser fluences where no morphological alterations were detected (30 MJ/cm²), high specific spectral variations were monitored to evaluate the cell photodegradation.
Sponsorship	IEEE Lasers and Electro-Optics Society
Starting Page	1
Ending Page	8
Page Count	8
File Size	3003902
File Format	PDF
ISSN	1077260X
Volume Number	22
Issue Number	3
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2016-01-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Laser beams Microscopy Image reconstruction Raman scattering Three-dimensional displays Lenses spermatozoa analysis Digital holography Raman spectroscopy
Content Type	Text
Resource Type	Article
Subject	Atomic and Molecular Physics, and Optics Electrical and Electronic Engineering

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