NDLI: A Simple and Robust Event-Detection Algorithm for Single-Cell Impedance Cytometry

Content Provider	IEEE Xplore Digital Library
Author	Caselli, F. Bisegna, P.
Copyright Year	1964
Abstract	Microfluidic impedance cytometry is emerging as a powerful label-free technique for the characterization of single biological cells. In order to increase the sensitivity and the specificity of the technique, suited digital signal processing methods are required to extract meaningful information from measured impedance data. In this study, a simple and robust event-detection algorithm for impedance cytometry is presented. Since a differential measuring scheme is generally adopted, the signal recorded when a cell passes through the sensing region of the device exhibits a typical odd-symmetric pattern. This feature is exploited twice by the proposed algorithm: first, a preliminary segmentation, based on the correlation of the data stream with the simplest odd-symmetric template, is performed; then, the quality of detected events is established by evaluating their E₂ O index, that is, a measure of the ratio between their even and odd parts. A thorough performance analysis is reported, showing the robustness of the algorithm with respect to parameter choice and noise level. In terms of sensitivity and positive predictive value, an overall performance of 94.9% and 98.5%, respectively, was achieved on two datasets relevant to microfluidic chips with very different characteristics, considering three noise levels. The present algorithm can foster the role of impedance cytometry in single-cell analysis, which is the new frontier in “Omics”.
Sponsorship	IEEE Engineering in Medicine and Biology Society
Starting Page	415
Ending Page	422
Page Count	8
File Size	728336
File Format	PDF
ISSN	00189294
Volume Number	63
Issue Number	2
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2016-01-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Impedance Electrodes Biomedical measurement Indexes Standards Signal to noise ratio Signal processing algorithms correlation single-cell analysis impedance cytometry event detection odd-symmetry Correlation
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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