NDLI: Dependence of the reversibility of focused- ultrasound-induced blood-brain barrier opening on pressure and pulse length in vivo

Content Provider	IEEE Xplore Digital Library
Author	Samiotaki, G. Konofagou, E.E.
Copyright Year	1986
Abstract	The most challenging aspect of intravenously-administered drugs currently developed to treat central nervous system (CNS) diseases is their impermeability through the blood-brain barrier (BBB), a specialized vasculature system protecting the brain microenvironment. Focused ultrasound (FUS) in conjunction with systemically administered microbubbles has been shown to open the BBB locally, noninvasively, and reversibly. The objective of this study was to investigate the effect of FUS (center frequency: 1.5 MHz) pulse length (PL), ranging here from 67 μs to 6.7 ms, on the physiology of the FUS-induced BBB opening. Dynamic contrastenhanced (DCE) and T1-weighted magnetic resonance imaging (MRI) were used to quantify the permeability changes using transfer rate (K_trans) mapping, the volume of BBB opening (VBBB) and the reversibility timeline of the FUS-induced BBB opening, with the systemic administration of microbubbles at different acoustic pressures, ranging from 0.30 to 0.60 MPa. Permeability and volume of opening were both found to increase with the acoustic pressure and pulse length. At 67-μs PL, the opening pressure threshold was 0.45 MPa, with BBB opening characteristics similar to those induced with 0.60 MPa at the same PL, as well as with 0.67-ms PL/0.30 MPa. On average, these cases had Ktrans = 0.0049 ± 0.0014 min-1 and V_BBB = 3.7 ± 4.3 mm³, and closing occurred within 8 h. The 6.7-ms PL/0.30 MPa induced similar opening with 0.67-ms PL/0.45 MPa, and a closing timeline of 24 to 48 h. On average, K_trans was 0.0091 ± 0.0029 min-1 and V_BBB was 14.13 ± 7.7 mm³ in these cases. Also, there were no significant differences between the 6.7-ms PL/0.45 MPa, 0.67-ms PL/0.60 MPa and 6.7-ms PL/0.60 MPa cases, yielding on average a K_trans of 0.0100 ± 0.0023 min-1 and V_BBB equal to 20.1 ± 5.7 mm³. Closing occurred within 48 to 72 h in these cases. Stacked histograms of the K_trans provided further insight to the nonuniform spatial distribution of permeability changes and revealed a correlation with the closing timeline. These results also suggest a beneficial complementary relationship between the elongation of the PL and the decrease of the peak negative acoustic pressures, and vice versa. Linear regression between K_trans and V_BBB showed a good correlation fit. Also, the time required for closing linearly increased with V_BBB. The volume rate of decrease was measured to be 11.4 ± 4.0 mm³ per day, suggesting that the closing timeline could be predicted from the initial volume of opening. Finally, no histological damage was detected in any of the cases 7 d post-FUS, indicating the safety of the methodology and parameters used.
Page Count	9
File Size	6225028
Starting Page	2257
Ending Page	2265
File Format	PDF
ISSN	08853010
Volume Number	60
Issue Number	11
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2013-01-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Acoustics Permeability Mice Magnetic resonance imaging Transducers Volume measurement Ultrasonic imaging
Content Type	Text
Resource Type	Article
Subject	Acoustics and Ultrasonics Instrumentation Electrical and Electronic Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Dependence of the Reversibility of Focused-Ultrasound-Induced Blood–Brain Barrier Opening on Pressure and Pulse Length In Vivo

Microbubble-Size Dependence of Focused Ultrasound-Induced Blood–Brain Barrier Opening in Mice In Vivo

A Quantitative Pressure and Microbubble-Size Dependence Study of Focused Ultrasound-Induced Blood-Brain Barrier Opening Reversibility in vivo Using MRI

In-vivo fetal ultrasound exposimetry

Numerical study of a simple transcranial focused ultrasound system applied to blood-brain barrier opening

Design and Experimental Evaluation of a 256-Channel Dual-Frequency Ultrasound Phased-Array System for Transcranial Blood–Brain Barrier Opening and Brain Drug Delivery

Effects of Ultrasound on Placental Function

Frequency dependence of speckle in continuous-wave ultrasound with implications for blood perfusion measurements

In vitro and in vivo brain ablation created by high-intensity focused ultrasound and monitored by MRI

Dependence of the reversibility of focused- ultrasound-induced blood-brain barrier opening on pressure and pulse length in vivo

Similar Documents

Dependence of the Reversibility of Focused-Ultrasound-Induced Blood–Brain Barrier Opening on Pressure and Pulse Length In Vivo

Microbubble-Size Dependence of Focused Ultrasound-Induced Blood–Brain Barrier Opening in Mice In Vivo

A Quantitative Pressure and Microbubble-Size Dependence Study of Focused Ultrasound-Induced Blood-Brain Barrier Opening Reversibility in vivo Using MRI

In-vivo fetal ultrasound exposimetry

Numerical study of a simple transcranial focused ultrasound system applied to blood-brain barrier opening

Design and Experimental Evaluation of a 256-Channel Dual-Frequency Ultrasound Phased-Array System for Transcranial Blood–Brain Barrier Opening and Brain Drug Delivery

Effects of Ultrasound on Placental Function

Frequency dependence of speckle in continuous-wave ultrasound with implications for blood perfusion measurements

In vitro and in vivo brain ablation created by high-intensity focused ultrasound and monitored by MRI

Dependence of the reversibility of focused- ultrasound-induced blood-brain barrier opening on pressure and pulse length in vivo