NDLI: Characterization of microbial communities found in bioreactor effluent

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Characterization of microbial communities found in bioreactor effluent

Content Provider	NASA Technical Reports Server (NTRS)
Author	Flowe, Candice
Copyright Year	2013
Description	The purpose of this investigation was to examine microbial communities of simulated wastewater effluent from hollow fiber membrane bioreactors collected from the Space Life Science Laboratory and Texas Technical University. Microbes were characterized using quantitative polymerase chain reaction where a total count of bacteria and fungi were determined. The primers that were used to determine the total count of bacteria and fungi were targeted for 16S rDNA genes and the internal transcribed spacer, respectively. PCR products were detected with SYBR Green I fluorescent dye and a melting curve analysis was performed to identify unique melt profiles resulting from DNA sequence variations from each species of the community. Results from both the total bacteria and total fungi count assays showed that distinct populations were present in isolates from these bioreactors. This was exhibited by variation in the number of peaks observed on the melting curve analysis graph. Further analysis of these results using species-specific primers will shed light on exactly which microbes are present in these effluents. Information gained from this study will enable the design of a system that can efficiently monitor microbes that play a role in the biogeochemical cycling of nitrogen in wastewater on the International Space Station to assist in the design of a sustainable system capable of converting this nutrient.
File Size	1994181
Page Count	6
File Format	PDF
Alternate Webpage(s)	http://archive.org/details/NASA_NTRS_Archive_20140002633
Archival Resource Key	ark:/13960/t6h181561
Language	English
Publisher Date	2013-07-22
Access Restriction	Open
Subject Keyword	Polymerase Chain Reaction Microorganisms Bioreactors Deoxyribonucleic Acid Effluents Assaying Waste Water Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science
Content Type	Text
Resource Type	Technical Report

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