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Microbial monitoring of common opportunistic pathogens by comparing multiple real-time pcr platforms for potential space applications
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Johnston, Angela S. Oubre, Cherie M. Roman, Monserrate C. Birmele, Michele N. Jett, Daniel X. Venkateswaran, Kasthuri J. Singhal, Adesh Jones, Kathy U. Ozbolt, Tamra A. Ott, C. Mark Vaishampayan, Parag A. Roberts, Michael S. Castro, Victoria A. |
| Copyright Year | 2013 |
| Description | Because the International Space Station is a closed environment with rotations of astronauts and equipment that each introduce their own microbial flora, it is necessary to monitor the air, surfaces, and water for microbial contamination. Current microbial monitoring includes labor- and time-intensive methods to enumerate total bacterial and fungal cells, with limited characterization, during in-flight testing. Although this culture-based method is sufficient for monitoring the International Space Station, on future long-duration missions more detailed characterization will need to be performed during flight, as sample return and ground characterization may not be available. At a workshop held in 2011 at NASA's Johnson Space Center to discuss alternative methodologies and technologies suitable for microbial monitoring for these long-term exploration missions, molecular-based methodologies such as polymerase chain reaction (PCR) were recommended. In response, a multi-center (Marshall Space Flight Center, Johnson Space Center, Jet Propulsion Laboratory, and Kennedy Space Center) collaborative research effort was initiated to explore novel commercial-off-the-shelf hardware options for space flight environmental monitoring. The goal was to evaluate quantitative or semi-quantitative PCR approaches for low-cost in-flight rapid identification of microorganisms that could affect crew safety. The initial phase of this project identified commercially available platforms that could be minimally modified to perform nominally in microgravity. This phase was followed by proof-of-concept testing of the highest qualifying candidates with a universally available challenge organism, Salmonella enterica. The analysis identified two technologies that were able to perform sample-to-answer testing with initial cell sample concentrations between 50 and 400 cells. In addition, the commercial systems were evaluated for initial flight safety and readiness. |
| File Size | 4171670 |
| Page Count | 12 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_20130013657 |
| Archival Resource Key | ark:/13960/t6256mj6k |
| Language | English |
| Publisher Date | 2013-01-01 |
| Access Restriction | Open |
| Subject Keyword | Real Time Pcr Platforms Salmonella Pathogens Spacecraft Cabin Atmospheres Spacecraft Space Laboratories Long Duration Space Flight Real Time Operation Commercial Off-the-shelf Products Polymerase Chain Reaction Microorganisms Closed Ecological Systems Proving Bacteria Environmental Monitoring Technology Utilization Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Article |
