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Species specific bacterial spore detection using lateral-flow immunoassay with dpa-triggered tb luminescence
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Ponce, Adrian |
| Copyright Year | 2003 |
| Description | A method of detecting bacterial spores incorporates (1) A method of lateral-flow immunoassay in combination with (2) A method based on the luminescence of Tb3+ ions to which molecules of dipicolinic acid (DPA) released from the spores have become bound. The present combination of lateral-flow immunoassay and DPA-triggered Tb luminescence was developed as a superior alternative to a prior lateral-flow immunoassay method in which detection involves the visual observation and/or measurement of red light scattered from colloidal gold nanoparticles. The advantage of the present combination method is that it affords both (1) High selectivity for spores of the species of bacteria that one seeks to detect (a characteristic of lateral-flow immunoassay in general) and (2) Detection sensitivity much greater (by virtue of the use of DPA-triggered Tb luminescence instead of gold nanoparticles) than that of the prior lateral-flow immunoassay method |
| File Size | 46977 |
| Page Count | 2 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_20110023718 |
| Archival Resource Key | ark:/13960/t73v4j158 |
| Language | English |
| Publisher Date | 2003-03-01 |
| Access Restriction | Open |
| Subject Keyword | Aerospace Medicine Immunoassay Acids Luminescence Bacteria Ions Terbium Biochemistry Detection Spores Bacteriology Microwaves Ntrs Nasa Technical Reports ServerĀ (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Technical Report |