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A gravity-responsive time-keeping protein of the plant and animal cell surface
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Morre Sr., D. James |
| Copyright Year | 2003 |
| Description | The hypothesis under investigation was that a ubiquinol (NADH) oxidase protein of the cell surface with protein disulfide-thiol interchange activity (= NOX protein) is a plant and animal time-keeping ultradian (period of less than 24 h) driver of both cell enlargement and the biological clock that responds to gravity. Despite considerable work in a large number of laboratories spanning several decades, this is, to my knowledge, our work is the first demonstration of a time-keeping biochemical reaction that is both gravity-responsive and growth-related and that has been shown to determine circadian periodicity. As such, the NOX protein may represent both the long-sought biological gravity receptor and the core oscillator of the cellular biological clock. Completed studies have resulted in 12 publications and two issued NASA-owned patents of the clock activity. The gravity response and autoentrainment were characterized in cultured mammalian cells and in two plant systems together with entrainment by light and small molecules (melatonin). The molecular basis of the oscillatory behavior was investigated using spectroscopic methods (Fourier transform infrared and circular dichroism) and high resolution electron microscopy. We have also applied these findings to an understanding of the response to hypergravity. Statistical methods for analysis of time series phenomena were developed (Foster et al., 2003). |
| File Size | 1323710 |
| Page Count | 22 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_20030056677 |
| Archival Resource Key | ark:/13960/t53f9qb3m |
| Language | English |
| Publisher Date | 2003-01-01 |
| Access Restriction | Open |
| Subject Keyword | Circadian Rhythms Gravitation Plants Botany Time Series Analysis Cell Membranes Biology Disulfides Oxidase Chemical Reactions Biochemistry Electron Microscopy Proteins Cells Biology Deoxyribonucleic Acid Oxidation Cloning Biology Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Technical Report |
