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Mechanical signal transduction in countermeasures to muscle atrophy
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Tidball, James G. |
| Copyright Year | 2002 |
| Description | We have shown that modifications in muscle use result in changes in the expression and activity of calpains and nitric oxide synthase (NOS). Although muscle unloading for 10 days produced no change in the concentrations of calpain 1 or 2 and no change in calpain activation, muscle reloading produced a 90% increase in calpain 2 concentration. We developed an in vitro model to test our hypothesis that nitric oxide can inhibit cytoskeletal breakdown in skeletal muscle cells by inhibiting calpain cleavage of talin. Talin was selected because it is a well-characterized calpain substrate and it is codistributed with calpain in muscle cells. We found that intermittant loading during hindlimb suspension that is sufficient to prevent muscle mass loss that occurs during muscle unloading is also sufficient to prevent the decrease in NOS expression that normally occurs during hindlimb unloading. These findings indicate that therapeutics directed toward regulating the calpain/calpastatin system may be beneficial in preventing muscle mass loss in muscle injury, unloading and disease. |
| File Size | 169433 |
| Page Count | 3 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_20020065430 |
| Archival Resource Key | ark:/13960/t5fc0158h |
| Language | English |
| Publisher Date | 2002-01-01 |
| Access Restriction | Open |
| Subject Keyword | Aerospace Medicine Unloading Muscles Nitrogen Oxides Hindlimb Suspension In Vitro Methods and Tests Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Technical Report |
