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T m -Shift Detection of Dog-Derived Ancylostoma ceylanicum and A. caninum
| Content Provider | Hindawi |
|---|---|
| Author | Fu, Yeqi Wang, Mingwei Yan, Xinxin Abdullahi, Auwalu Yusuf Hang, Jianxiong Zhang, Pan Huang, Yue Liu, Yunqiu Sun, Yongxiang Ran, Rongkun Li, Guoqing |
| Editor | Mancianti, Francesca |
| Copyright Year | 2018 |
| Abstract | To develop a Tm-shift method for detection of dog-derived Ancylostoma ceylanicum and A. caninum, three sets of primers were designed based on three SNPs (ITS71, ITS197, and ITS296) of their internal transcribed spacer 1 (ITS1) sequences. The detection effect of the Tm-shift was assessed through the stability, sensitivity, accuracy test, and clinical detection. The results showed that these three sets of primers could distinguish accurately between A. ceylanicum and A. caninum. The coefficient of variation in their Tm values on the three SNPs was 0.09% and 0.15% (ITS71), 0.18% and 0.14% (ITS197), and 0.13% and 0.07% (ITS296), respectively. The lowest detectable concentration of standard plasmids for A. ceylanicum and A. caninum was 5.33 × 10−6 ng/μL and 5.03 × 10−6 ng/μL. The Tm-shift results of ten DNA samples from the dog-derived hookworms were consistent with their known species. In the clinical detection of 50 fecal samples from stray dogs, the positive rate of hookworm detected by Tm-shift (42%) was significantly higher than that by microscopic examination (34%), and the former can identify the Ancylostoma species. It is concluded that the Tm-shift method is rapid, specific, sensitive, and suitable for the clinical detection and zoonotic risk assessment of the dog-derived hookworm. |
| Page Count | 8 |
| File Format | |
| ISSN | 23146133 |
| DOI | 10.1155/2018/7617094 |
| Journal | BioMed Research International |
| Volume Number | 2018 |
| e-ISSN | 23146141 |
| Language | English |
| Publisher | Hindawi |
| Publisher Date | 2018-05-13 |
| Access Restriction | Open |
| Rights License | This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology |
