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BioSAXS at European Synchrotron Radiation Facility – Extremely Brilliant Source: BM29 with an upgraded source, detector, robot, sample environment, data collection and analysis software
| Content Provider | Hyper Articles en Ligne (HAL) |
|---|---|
| Author | Tully, Mark, D Kieffer, Jerome Brennich, Martha, E Cohen Aberdam, Raphael Florial, Jean Baptiste Hutin, Stephanie Oscarsson, Markus Beteva, Antonia Popov, Anton Moussaoui, Dihia Theveneau, Pascal Papp, Gergely Gigmes, Jonathan Cipriani, Florent Mccarthy, Andrew Zubieta, Chloe Mueller-Dieckmann, Christoph Leonard, Gordon Pernot, Petra |
| Copyright Year | 2023 |
| Abstract | As part of its Extremely Brilliant Source (EBS) upgrade project, the ESRF's BM29 BioSAXS beamline was subject to a significant upgrade and refurbishment. In addition to the replacement of the beamline's original bending magnet source by a two-pole wiggler, leading to an increase in brilliance by a factor of 60, the sample environment of the beamline was almost completely refurbished: a vacuum-compatible Pilatus3 X 2M with a sensitive area of 253.7 mm × 288 mm and frame rates up to 250 Hz was installed, increasing the active area available and thus the q -scaling of scattering images taken; the sample changer was replaced with an upgraded version, allowing more space for customizable sample environments and the installation of two new sample exposure units; the software associated with the beamline was also renewed. In addition, the layout and functionality of the BSXCuBE3 ( BioSAXS Customized Beamline Environment ) data acquisition software was redesigned, providing an intuitive `user first' approach for inexperienced users, while at the same time maintaining more powerful options for experienced users and beamline staff. Additional features of BSXCuBE3 are queuing of samples; either consecutive sample changer and/or SEC-SAXS (size-exclusion chromatography small-angle X-ray scattering) experiments, including column equilibration were also implemented. Automatic data processing and analysis are now managed via Dahu , an online server with upstream data reduction, data scaling and azimuthal integration built around PyFAI ( Python Fast Azimuthal Integration ), and data analysis performed using the open source FreeSAS . The results of this automated data analysis pipeline are displayed in ISPyB/ExiSAXS . The upgraded BM29 has been in operation since the post-EBS restart in September 2020, and here a full description of its new hardware and software characteristics together with examples of data obtained are provided. |
| Related Links | https://hal.science/hal-03942822/file/s-30-00258.pdf |
| ISSN | 09090495 |
| e-ISSN | 16005775 |
| DOI | 10.1107/S1600577522011286 |
| Journal | Journal of Synchrotron Radiation |
| PubMed reference number | 36601945 |
| Issue Number | 1 |
| PubMed Central reference number | PMC9814054 |
| Volume Number | 30 |
| Language | English |
| Publisher | HAL CCSD International Union of Crystallography |
| Publisher Date | 2023-01-01 |
| Access Restriction | Open |
| Subject Keyword | BM29 high-throughput small-angle X-ray scattering SAXS macromolecules in solution online size-exclusion chromatography (SEC-SAXS) microfluidic screening macromolecules in solution online size-exclusion chromatography (SEC-SAXS high-throughput small-angle X-ray scattering microfluidic screening BM29 SAXS |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nuclear and High Energy Physics Instrumentation Biochemistry, Genetics and Molecular Biology Radiation |
