NDLI: Imaging of extracellular cathepsin S activity by a selective near infrared fluorescence substrate-based probe

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Imaging of extracellular cathepsin S activity by a selective near infrared fluorescence substrate-based probe

Content Provider	Hyper Articles en Ligne (HAL)
Author	Wartenberg, Mylène Saidi, Ahlame Galibert, Mathieu Joulin-Giet, Alix Burlaud-Gaillard, Julien Lecaille, Fabien Scott, Christopher Aucagne, Vincent Delmas, A.F. Lalmanach, Gilles
Copyright Year	2019
Abstract	We designed a near-infrared fluorescent substrate-based probe (SBP), termed MG101, for monitoring extracellular cathepsin S (CatS) activity. We conceived a fused peptide hairpin loop-structure, combining a CatS recognition domain, an electrostatic zipper (with complementary charges of a polyanionic (D-Glu)5 segment and a polycationic (D-Arg)5 motif, as well as a N and C terminal Förster resonance energy transfer pair (donor: AlexaFluor680; quencher: BHQ3) to facilitate activity-dependent imaging. MG101 showed excellent stability since no fluorescence release corresponding to a self-dequenching was observed in the presence of either 2 M NaCl or after incubation at a broad range of pH (2.2-8.2). Cathepsins B, D, G, H, and K, neutrophil elastase and proteinase 3 did not cleave MG101, while CatS, and to a lesser extent CatL, hydrolysed MG101 at pH 5.5. However MG101 was fully selective for CatS at pH 7.4 (kcat/Km = 140,000 M-1 s-1) and sensitive to low concentration of CatS (
Related Links	https://hal.science/hal-02088627/file/S0300908419300823.pdf
ISSN	03009084
DOI	10.1016/j.biochi.2019.03.013
Journal	Biochimie
PubMed reference number	30914255
Volume Number	166
Language	English
Publisher	HAL CCSD Elsevier
Publisher Date	2019-11-01
Access Restriction	Open
Subject Keyword	Cysteine cathepsin Förster resonance energy transfer (FRET Near-infrared fluorescence (NIRF Protease Macrophage Life Sciences [q-bio] Biochemistry [q-bio.BM] Life Sciences [q-bio] Biochemistry, Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry

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