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Ligand-binding properties of a juvenile hormone receptor, Methoprene-tolerant.
| Content Provider | Hyper Articles en Ligne (HAL) |
|---|---|
| Author | Charles, Jean-Philippe Iwema, Thomas Epa, V Chandana Takaki, Keiko Rynes, Jan Jindra, Marek |
| Copyright Year | 2011 |
| Abstract | Juvenile hormone (JH) is a sesquiterpenoid of vital importance for insect development, yet the molecular basis of JH signaling remains obscure, mainly because a bona fide JH receptor has not been identified. Mounting evidence points to the basic helix-loop-helix (bHLH)/Per-Arnt-Sim (PAS) domain protein Methoprene-tolerant (Met) as the best JH receptor candidate. However, details of how Met transduces the hormonal signal are missing. Here, we demonstrate that Met specifically binds JH III and its biologically active mimics, methoprene and pyriproxyfen, through its C-terminal PAS domain. Substitution of individual amino acids, predicted to form a ligand-binding pocket, with residues possessing bulkier side chains reduces JH III binding likely because of steric hindrance. Although a mutation that abolishes JH III binding does not affect a Met-Met complex that forms in the absence of methoprene, it prevents both the ligand-dependent dissociation of the Met-Met dimer and the ligand-dependent interaction of Met with its partner bHLH-PAS protein Taiman. These results show that Met can sense the JH signal through direct, specific binding, thus establishing a unique class of intracellular hormone receptors. |
| Related Links | https://hal.science/hal-00824822/file/Ligand_binding_properties.pdf |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| DOI | 10.1073/pnas.1116123109 |
| PubMed reference number | 22167806 |
| Issue Number | 52 |
| Volume Number | 108 |
| Conference Proceedings | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Publisher | HAL CCSD National Academy of Sciences |
| Publisher Date | 2011-01-01 |
| Access Restriction | Open |
| Subject Keyword | transcription factor molecular actions expression Drosophila insecticide action regulators structure modeling drosophila-melanogaster proteins beetle tribolium-castaneum metamorphosis Tribolium |
| Content Type | Text |
| Resource Type | Conference Proceedings |
| Subject | Biochemistry, Genetics and Molecular Biology Multidisciplinary |
