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| Content Provider | frontiers |
|---|---|
| Author | Shahid, Muhammad Shafiq Sattar, Muhammad Naeem Iqbal, Zafar Raza, Amir Al-Sadi, Abdullah M. |
| Description | In recent years, next-generation sequencing (NGS) and contemporary Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated (Cas) technologies have revolutionized the life sciences and the field of plant virology. Both these technologies offer an unparalleled platform for sequencing and deciphering viral metagenomes promptly. Over the past two decades, NGS technologies have improved enormously and have impacted plant virology. NGS has enabled the detection of plant viruses that were previously undetectable by conventional approaches, such as quarantine and archeological plant samples, and has helped to track the evolutionary footprints of viral pathogens. The CRISPR-Cas-based genome editing (GE) and detection techniques have enabled the development of effective approaches to virus resistance. Different versions of CRISPR-Cas have been employed to successfully confer resistance against diverse plant viruses by directly targeting the virus genome or indirectly editing certain host susceptibility factors. Applications of CRISPR-Cas systems include targeted insertion and/or deletion, site-directed mutagenesis, induction/expression/repression of the gene(s), epigenome re-modeling, and SNPs detection. The CRISPR-Cas toolbox has been equipped with precision GE tools to engineer the target genome with and without double-stranded (ds) breaks or donor templates. This technique has also enabled the generation of transgene-free genetically engineered plants... |
| Abstract | Next-generation sequencing (NGS) and contemporary CRISPR-Cas technologies have revolutionized all the fields of life sciences and plant virology is not an exception. Both these technilofies offer an unparalleled platform to sequencing and deciphering of viral metagenomes promptly Over the past couple of decades, NGS technologies have improved enormously and have impacted the field of plant virology by prompt sequencing, unbiased and hypothesis-free testing and deciphering viral metagenomes. NGS has enabled the detection of plant viruses that had remained undetectable by conventional approaches, such as quarantine and archeological plant samples, and has helped to track the evolutionary footprints of viral pathogens. The CRISPR-Cas based genome editing (GE) and detection techniques have funneled the development of effective virus resistance approaches. Different versions of CRISPR-Cas have been employed successfully to confer resistance against diverse plant viruses by directly targeting the virus genome or indirectly editing certain host susceptibility factors. Applications of CRISPR-Cas systems include targeted insertion and/or deletion, site-directed mutagenesis, induction/expression/repression of the gene(s), epigenome re-modeling and SNPs detection. The CRISPR-Cas toolbox has been equipped with precision GE tools to engineer the target genome with and without double-stranded breaks or donor templates. This technique also enabled the generation of transgene-free genetically engineered plants, DNA repair, base substitution, prime editing, detection of small molecules, and biosensing in plant virology. The utilities, advantages, applications, bottlenecks of NGS and CRISPR-Cas in plant virology are discussed in this review. |
| ISSN | 1664302X |
| DOI | 10.3389/fmicb.2020.609376 |
| Volume Number | 11 |
| Journal | Frontiers in Microbiology |
| Language | English |
| Publisher Date | 2021-01-12 |
| Access Restriction | Open |
| Subject Keyword | CRISPR Plant Viruses Genome editing CRISPR associated (Cas) proteins Next generation sequencing (NGS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology Microbiology (medical) |
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