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| Content Provider | frontiers |
|---|---|
| Author | Svandova, Eva Vesela, Barbora Tucker, Abigail S. Matalova, Eva |
| Abstract | Caspases are proteases well known in the context of inflammation and apoptosis. Recently, novel roles of pro-apoptotic caspases have been reported including findings related to the development of hard tissues. To further investigate these emerging functions of pro-apoptotic caspases, the in vivo localization of key pro-apoptotic caspases (-3, -6, -7, -8 and -9) was assessed, concentrating on the development of two neighbouring hard tissues, cells participating in odontogenesis (represented by the first mouse molar) and intramembranous osteogenesis (mandibular/alveolar bone). The expression of the different caspases within the developing tissues was correlated with the apoptotic status of the cells, to produce a picture of whether different caspases have potentially distinct or overlapping non-apoptotic functions. The in vivo investigation was additionally supported by examination of caspases in an osteoblast-like cell line in vitro. Caspase-3, -7 and -9 were activated in apoptotic cells of the primary enamel knot of the first molar, however, caspase-7 and -8 activation was associated also with the non-apoptotic enamel epithelium at the same stage and later with differentiating/differentiated odontoblasts and ameloblasts. In the adjacent bone, active caspase-7 and -8 were present abundantly in the prenatal period, while the appearance of caspase-3, -6 and -9 was marginal. Perinatally, caspase-3 and -7 were evident in some osteoclasts and osteoblastic cells and caspase-8 was abundant mostly in osteoclasts. In addition, postnatal activation of caspase-7 and -8 was retained in osteocytes. The results provide a comprehensive temporo-spatial pattern of pro-apoptotic caspase activation, and demonstrate both unique and overlapping activation in non-apoptotic cells during development of the molar tooth and mandibular/alveolar bone. The importance of caspases in osteogenic pathways is highlighted by caspase inhibition in osteoblast-like cells, which led to a significant decrease in osteocalcin expression, supporting a role in hard tissue cell differentiation. |
| ISSN | 1664042X |
| DOI | 10.3389/fphys.2018.00174 |
| Volume Number | 9 |
| Journal | Frontiers in Physiology |
| Language | English |
| Publisher Date | 2018-03-07 |
| Access Restriction | Open |
| Subject Keyword | Tooth Bone Differentiation Apoptosis Caspase Intramembranous Osteocalcin |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Physiology (medical) |
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