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| Content Provider | frontiers |
|---|---|
| Author | Yan, Fengna Zhang, Qun Shi, Ke Zhang, Yi Zhu, Bingbing Bi, Yufei Wang, Xianbo |
| Description | Background and aimsGiven hepatitis B virus (HBV)-related hepatocellular carcinoma (HBV-HCC) exhibits unique gut microbiota characteristics and a significant immunosuppressive tumor microenvironment. Thus, a better understanding of the correlation between gut microbiota and the immunosuppressive response may help predict occurrence and prognosis of HBV-HCC.MethodsHere, in a cohort of ninety adults (healthy control n=30, HBV-cirrhosis n=30, HBV-HCC n=30) with clinical data, fecal 16S rRNA gene sequencing, matched peripheral blood immune response with flow cytometry analysis. Correlation between the gut microbiome of significantly different in HBV-HCC patients and clinical parameters as well as the peripheral immune response was assessed.ResultsWe found that community structures and diversity of the gut microbiota in HBV-CLD patients become more unbalanced. Differential microbiota analysis that p:Acidobacteriota, p:Proteobacteria, p:Campilobacterota, f:Streptococcaceae, g:Klebsiella associated with inflammation were enriched. The beneficial bacteria of f:Clostridia UCG−014, f:Oscillospiraceae, f:_Rikenellaceae, g:_Barnesiella, g:Prevotella, g:Agathobacter were decreased. Functional analysis of gut microbiota revealed that lipopolysaccharide biosynthesis, lipid metabolism, butanoate metabolism were significantly elevated in HBV-CLD patients. Spearman’s correlation analysis showed that Muribaculaceae, Akkermaniacaeae, [Eubacterium]_coprostanoligenes_group, RF39, Tannerellaceae ... |
| Abstract | ABSTRACT Background and aims: Given hepatitis B virus (HBV)-related hepatocellular carcinoma (HBV-HCC) exhibits unique gut microbiota characteristics and a significant immunosuppressive tumor microenvironment. Thus, a better understanding of the correlation between gut microbiota and the immunosuppressive response may help predict occurrence and prognosis of HBV-HCC. Methods: Ninety clinical samples (healthy control n=30, HBV-cirrhosis n=30, HBV-HCC n=30) were studied. We used the 16S rRNA gene sequencing and flow cytometry to characterize gut microbiota and peripheral immune response of HBV related chronic liver disease (HBV-CLD) patients. Correlation between the gut microbiome and clinical parameters as well as between the gut microbiome and the peripheral immune response of participants was assessed. Results: Compared with the healthy group, the abundance of Firmicutes and Bacteroidota was significantly decreased in HBV-CLD patients, yet Proteobacteria and Actinobacteriota were elevated. Diversity of the gut microbiome was reduced as the disease progressesand. The immunosuppressive response of HBV-HCC patients was stronger compared with HBV-cirrhosis (HBV-LC) patients. Furthermore, The percentage of CD3+T, CD4+T and CD8+T cells were positively correlated with the abundance of Bacteroidota, Deferribacterota, and Akkermaniacaeae. The percentage of Treg cell and programmed cell death 1 (PD-1), cytotoxic T-lymphocyte antigen 4 (CTLA-4), immune receptor tyrosine based inhibitor motor (ITIM) domain (TIGIT), T-cell immune domain, and multiple domain 3 (TIM-3) in HBV-HCC patients were positively correlated with harmful bacteria, such as Actinobaciota, Myxococota, Streptococcaceae and Eubacterium coprostanoligenes. Conclusions: Our study indicated that the dysbiosis of microbiota was associated with the peripheral T cell immune response in HBV-CLD patients. Keywords: Gut microbiome, liver disease, T cell immunosuppression |
| ISSN | 22352988 |
| DOI | 10.3389/fcimb.2023.1152987 |
| Volume Number | 13 |
| Journal | Frontiers in Cellular and Infection Microbiology |
| Language | English |
| Publisher Date | 2023-05-02 |
| Access Restriction | Open |
| Subject Keyword | Gut microbiome Liver disease Hepatocellular Carcinoma Peripheral immune response T cell immunosuppression |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Immunology Microbiology Microbiology (medical) |
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