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| Content Provider | frontiers |
|---|---|
| Author | Wu, Jing Jin, Ying-Ying Gong, Ruo-Lan Yang, Fan Su, Xiao-Ya Chen, Tong-Xin |
| Abstract | Toll like receptors (TLRs) induced response plays a vital role in B-cell development and activation, in which TLR7-mediated and TLR9-mediated response interact together and play antagonistic or cooperative roles at different situation. Previous studies showed that the transcription factor signal transducer and activator of transcription (STAT) 3 was one of the key transcriptional factors (TFs) needed for both TLR7 and TLR9 signaling in B-cell, and patients with autosomal dominant hyper IgE syndromes (AD-HIES) due to STAT3 mutations had defective TLRs response in B cells. However, how STAT3 affects its target genes as well as the downstream signaling pathways in B-cell upon TLRs stimulation remains unclarified on a genome-wide level. ChIP-seq and RNA-seq was used in this study to identify the STAT3 targets in response TLRs stimulation in human B-cell. STAT3 ChIP-seq results showed a total of 611 and 2289 differential STAT3-binding sites were identified in human B-cell after TLR7 and TLR9 agonists stimulation, respectively. RNA-seq results showed 1186 and 1775 differentially expressed genes after TLR7 and TLR9 activation, respectively. We identified 47 primary STAT3 target genes after TLR7 activation and 189 target genes after TLR9 activation in B-cell by integration of STAT3 ChIP-seq and RNA-seq data. Among these STAT3 primary targets, we identified 7 TFs and 18 TFs for TLR7 and TLR9 response, respectively. Besides, we showed that STAT3 might regulate TLR9, but not TLR7 response in B-cell through directly regulating integrin signaling pathway, which might further affect the antagonism between TLR7 and TLR9 signaling in B-cell. Our study provides insights into the molecular mechanism of human TLRs response in B-cell and how it can be regulated, which provides a better understanding and modulating TLR-mediated pathogenic immune responses in B-cell. |
| ISSN | 16643224 |
| DOI | 10.3389/fimmu.2022.821457 |
| Volume Number | 13 |
| Journal | Frontiers in Immunology |
| Language | English |
| Publisher Date | 2022-03-08 |
| Access Restriction | Open |
| Subject Keyword | Stat3 Toll-Like Receptors ChIP sequencing B cells RNA sequencing |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
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