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| Content Provider | frontiers |
|---|---|
| Author | Yan, Guangwen Li, Shuaibing Wen, Yuhang Luo, Yadan Huang, Jingrong Chen, Baoting Lv, Shuya Chen, Lang He, Lvqin He, Manli Yang, Qian Yu, Zehui Xiao, Wudian Tang, Yong Li, Weiyao Han, Jianhong Zhao, Fangfang Yu, Shumin Kong, Fang Abbasi, Benazir Yin, Hongmei Gu, Congwei |
| Description | IntroductionAs a representation of the gut microbiota, fecal and cecal samples are most often used in human and animal studies, including in non-alcoholic fatty liver disease (NAFLD) research. However, due to the regional structure and function of intestinal microbiota, whether it is representative to use cecal or fecal contents to study intestinal microbiota in the study of NAFLD remains to be shown.MethodsThe NAFLD mouse model was established by high-fat diet induction, and the contents of the jejunum, ileum, cecum, and colon (formed fecal balls) were collected for 16S rRNA gene analysis.ResultsCompared with normal mice, the diversity and the relative abundance of major bacteria and functional genes of the ileum, cecum and colon were significantly changed, but not in the jejunum. In NAFLD mice, the variation characteristics of microbiota in the cecum and colon (feces) were similar. However, the variation characteristics of intestinal microbiota in the ileum and large intestine segments (cecum and colon) were quite different.DiscussionTherefore, the study results of cecal and colonic (fecal) microbiota cannot completely represent the results of jejunal and ileal microbiota. |
| Abstract | As a representation of the gut microbiota, fecal and cecal samples are most often used in human and animal studies, including in non-alcoholic fatty liver disease (NAFLD) research. However, due to the regional structure and function of intestinal microbiota, whether it is representative to use cecal or fecal contents to study intestinal microbiota in the study of NAFLD remains to be shown. The NAFLD mouse model was established by high-fat diet induction, and the contents of the jejunum, ileum, cecum, and colon were collected for 16S rDNA analysis. Colonic contents (formed fecal balls) were used instead of feces due to the uncertainty of fecal sample collection and the high similarity of fecal and colonic contents microbiota in mice. Compared with normal mice, the diversity and the relative abundance of major microbiota and functional genes of the ileum, cecum and colon were significantly changed, but not in the jejunum. The variation characteristics of microbiota in the cecum and colon (feces) were similar. However, the variation characteristics of intestinal microbiota in the ileum and large intestine segments (cecum and colon) were quite different. Therefore, the study results of cecal and colonic (fecal) microbiota cannot completely represent the results of jejunal and ileal microbiota. |
| ISSN | 1664302X |
| DOI | 10.3389/fmicb.2022.1051200 |
| Volume Number | 13 |
| Journal | Frontiers in Microbiology |
| Language | English |
| Publisher Date | 2022-12-22 |
| Access Restriction | Open |
| Subject Keyword | Intestinal microbiota Mice 16s rDNA sequencing Nonalcoholic fatty liver disease (NAFLD) High fat diet (HFD) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology Microbiology (medical) |
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