NDLI: Proteomics as the final step in the functional metagenomics study of antimicrobial resistance

Content Provider	frontiers
Author	Fouhy, Fiona Stanton, Catherine Cotter, Paul D. Hill, Colin Walsh, Fiona
Abstract	The majority of clinically applied antimicrobial agents are derived from natural products generated by soil microorganisms and therefore resistance is likely to be ubiquitous in such environments. This is supported by the fact that numerous clinically important resistance mechanisms are encoded within the chromosomes of such bacteria. Advances in genomic sequencing have enabled the in silico identification of putative resistance genes present in these microorganisms. However, it is not sufficient to rely on the identification of putative resistance genes, we must also determine if the resultant proteins confer a resistant phenotype. This will require an analysis pipeline that extends from the extraction of environmental DNA, to the identification and analysis of potential resistance genes and their resultant proteins and phenotypes. This review focuses on the application of functional metagenomics and proteomics to study antimicrobial resistance in diverse environments.
ISSN	1664302X
DOI	10.3389/fmicb.2015.00172
Volume Number	6
Journal	Frontiers in Microbiology
Language	English
Publisher Date	2015-03-03
Access Restriction	Open
Subject Keyword	Novel mechanisms Microbiology Antibiotic resistance Proteins Functional metagenomics
Content Type	Text
Resource Type	Article
Subject	Microbiology Microbiology (medical)

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Proteomics as the final step in the functional metagenomics study of antimicrobial resistance