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| Content Provider | frontiers |
|---|---|
| Author | He, Yuxi Guo, Wenyu Peng, Jieli Guo, Jinying Ma, Jia Wang, Xu Zhang, Cuimian Jia, Nan Wang, Entao Hu, Dong Wang, Zhanwu |
| Description | To investigate the mechanism underlying the plant growth-promoting (PGP) effects of strain Streptomyces sp. TOR3209, PGP traits responsible for indoleacetic acid production, siderophore production, and phosphate solubilization were tested by culturing the strain TOR3209 in the corresponding media. The effects of volatile organic compounds (VOCs) produced by the strain TOR3209 on plant growth were observed by co-culturing this strain with tobacco seedlings in I-plates. Meanwhile, the effects of VOCs on tobacco gene expression were estimated by performing a transcriptome analysis, and VOCs were identified by the solid-phase micro-extraction (SPME) method. The results showed positive reactions for the three tested PGP traits in the culture of strain TOR3209, while the tobacco seedlings co-cultured with strain TOR3209 revealed an increase in the fresh weight by up to 100% when compared to that of the control plants, demonstrating that the production VOCs was also a PGP trait. In transcriptome analysis, plants co-cultured with strain TOR3209 presented the highest up-regulated expression of the genes involved in plant growth and development processes, implying that the bacterial VOCs played a role as a regulator of plant gene expression. Among the VOCs produced by the strain TOR3209, two antifungal molecules, 2,4-bis(1,1-dimethylethyl)-phenol and hexanedioic acid dibutyl ester, were found as the main compounds. Conclusively, up-regulation in the expression of growth- and develop... |
| Abstract | The PGPR strain Streptomyces sp. TOR3209 isolated from rhizosphere of tomato could increase disease resistance, promote growth of several plants, and modify the rhizosphere microbial community. However, the mechanism of these effects maintains unclear and the growth promotion by directly inoculating this strain to plant roots was not significant in some cases. Considering the regulatory role of microbial volatile organic compounds (VOCs) in plant growth and development, we hypothesize that the strain TOR3209 might benefit the plants by producing VOCs. To verify this hypothesis, VOCs produced by TOR3209 were collected and used to treat the tobacco and tomato seedlings. The results proofed that the TOR3209 VOCs increased the fresh weight of tobacco in I-plate up to 119% than that of CK. In pot experiment with miniature greenhouse, the VOCs increased the total dry weight of tomato about 39%, and promoted the root development, compared with that in CK. Remarkably, study on transcriptional changes in plants treated with the VOCs revealed that the highest gene differential expression was observed on UDP-glycosyltransferase, Glutamate receptor, RING/U-box protein with C6HC-type zinc finger and Leucine-rich repeat receptor-like protein kinase family protein, all being involved in plant growth and development processes. By solid phase micro extraction (SPME) method, 2,4-bis(1,1-dimethylethyl)-phenol and hexanedioic acid dibutyl ester were found as the main VOCs produced by TOR3209. In this study, we revealed that the VOCs produced by strain TOR3209 could regulate plant growth in a non-contact manner, and further efforts will be made to explore the effective VOCs in biological promotion. |
| ISSN | 1664302X |
| DOI | 10.3389/fmicb.2022.891245 |
| Volume Number | 13 |
| Journal | Frontiers in Microbiology |
| Language | English |
| Publisher Date | 2022-05-20 |
| Access Restriction | Open |
| Subject Keyword | Streptomyces SPME RNA squencing Volatile organic compounds(VOCs) PGPR - plant growth-promoting rhizobacteria |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology Microbiology (medical) |
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