DNA methylation is enhanced during Cd hyperaccumulation in Noccaea caerulescens ecotype Ganges.

Content Provider	Europe PMC
Author	Galati, Serena DalCorso, Giovanni Furini, Antonella Fragni, Rosaria Maccari, Chiara Mozzoni, Paola Giannelli, Gianluigi Buschini, Annamaria Visioli, Giovanna
Abstract	In this study, we assess the DNA damage occurring in response to cadmium (Cd) in the Cd hyperaccumulator Noccaea caerulescens Ganges (GA) vs the non-accumulator and close-relative species Arabidopsis thaliana. At this purpose, the alkaline comet assay was utilized to evaluate the Cd-induced variations in nucleoids and the methy-sens comet assay, and semiquantitative real-time (qRT)-PCR were also performed to associate nucleus variations to possible DNA modifications. Cadmium induced high DNA damages in nuclei of A. thaliana while only a small increase in DNA migration was observed in N. caerulescens GA. In addition, in N. caerulescens GA, CpG DNA methylation increase upon Cd when compared to control condition, along with an increase in the expression of MET1 gene, coding for the DNA-methyltransferase. N. caerulescens GA does not show any oxidative stress under Cd treatment, while A. thaliana Cd-treated plants showed an upregulation of transcripts of the respiratory burst oxidase, accumulation of reactive oxygen species, and enhanced superoxide dismutase activity. These data suggest that epigenetic modifications occur in the N. caerulescens GA exposed to Cd to preserve genome integrity, contributing to Cd tolerance.Supplementary InformationThe online version contains supplementary material available at 10.1007/s11356-022-23983-w.
ISSN	09441344
Volume Number	30
PubMed Central reference number	PMC9995422
Issue Number	10
PubMed reference number	36352075
Journal	Environmental Science and Pollution Research International [Environ Sci Pollut Res Int]
e-ISSN	16147499
DOI	10.1007/s11356-022-23983-w
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2022-11-10
Publisher Place	Berlin/Heidelberg
Access Restriction	Open
Rights License	Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2022
Subject Keyword	Hyperaccumulators Noccaea caerulescens Cadmium toxicity Comet assay Methy-sens comet assay Antioxidant activity Epigenetic regulation
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Pollution Health, Toxicology and Mutagenesis Medicine