NDLI: Cytotoxic Isopentenyl Phloroglucinol Compounds from <i>Garcinia xanthochymus</i> Using LC-MS-Based Metabolomics.

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Cytotoxic Isopentenyl Phloroglucinol Compounds from Garcinia xanthochymus Using LC-MS-Based Metabolomics.

Content Provider	Europe PMC
Author	Quan, Fan Luan, Xinbo Zhang, Jian Gao, Wenjie Yan, Jian Li, Ping
Editor	Gu, Ronghui Gu, Wei Ji, Yuanyuan
Copyright Year	2023
Abstract	Many unique chemical metabolites with significant antitumor activities have been isolated from Garcinia species and have become a leading hotspot of antitumor research in recent years. The aim of this study was to identify bioactive compounds from different plant parts (leaf, branch, stem bark, fruit, and seed) of G. xanthochymus through combining LC-MS-based metabolomics with cytotoxicity assays. As a result, 70% methanol seed extract exerted significant cytotoxic effects on five human cancer cell types (HL-60, A549, SMMC-7721, MDA-MB-231, and SW480). LC-MS-based metabolomics analysis was used, including principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), in order to identify 12 potential markers from seed extract that may relate to bioactivity. LC-MS guidance isolated the markers to obtain three compounds and identified new isopentenyl phloroglucinols (1–3, named garxanthochin A–C), using spectroscopic methods. Among them, garxanthochin B (2) demonstrated moderate inhibitory activities against five human cancer cell types, with IC50 values of 14.71~24.43 μM. These findings indicate that G. xanthochymus seed has significant cytotoxic activity against cancer cells and garxanthochin B has potential applications in the development of antitumor-led natural compounds.
Volume Number	13
PubMed Central reference number	PMC9964469
Issue Number	2
PubMed reference number	36837877
Journal	Metabolites
e-ISSN	22181989
DOI	10.3390/metabo13020258
Language	English
Publisher	MDPI
Publisher Date	2023-02-10
Access Restriction	Open
Rights License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors.
Subject Keyword	Garcinia xanthochymus metabolomics cytotoxicity biomarker LC-MS isopentenyl phloroglucinols
Content Type	Text
Resource Type	Article
Subject	Biochemistry Endocrinology, Diabetes and Metabolism Molecular Biology

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