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The Colorimetric Detection of the Hydroxyl Radical.
| Content Provider | Europe PMC |
|---|---|
| Author | Ran, Yandong Moursy, Mohammed Hider, Robert C. Cilibrizzi, Agostino |
| Editor | Bobrowski, Krzysztof |
| Copyright Year | 2023 |
| Abstract | An aromatic substrate for hydroxylation by hydroxyl radicals (•OH) was investigated. The probe, N,N'-(5-nitro-1,3-phenylene)-bis-glutaramide, and its hydroxylated product do not bind either iron(III) or iron(II), and so they do not interfere with the Fenton reaction. A spectrophotometric assay based on the hydroxylation of the substrate was developed. The synthesis and purification methods of this probe from previously published methodologies were improved upon, as well as the analytical procedure for monitoring the Fenton reaction through its use, enabling univocal and sensitive •OH detection. The assay was utilised to demonstrate that the iron(III) complexes of long-chain fatty acids lack Fenton activity under biological conditions. |
| Journal | International Journal of Molecular Sciences [Int J Mol Sci] |
| Volume Number | 24 |
| DOI | 10.3390/ijms24044162 |
| PubMed Central reference number | PMC9964436 |
| Issue Number | 4 |
| PubMed reference number | 36835574 |
| e-ISSN | 14220067 |
| Language | English |
| Publisher | Molecular Diversity Preservation International (MDPI) |
| Publisher Date | 2023-02-19 |
| Access Restriction | Open |
| Subject Keyword | hydroxyl radical iron complexes fatty acids aromatic hydroxylation Fenton reaction Fenton activity |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physical and Theoretical Chemistry Molecular Biology Spectroscopy Catalysis Computer Science Applications Medicine Inorganic Chemistry Organic Chemistry |
