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miR-199a-5p Reduces Chondrocyte Hypertrophy and Attenuates Osteoarthritis Progression via the Indian Hedgehog Signal Pathway.
| Content Provider | Europe PMC |
|---|---|
| Author | Huang, Lei Jin, Meng Gu, Ruiying Xiao, Kunlin Lu, Mengnan Huo, Xinyu Sun, Mengyao Yang, Zhi Wang, Zhiyuan Zhang, Weijie Zhi, Liqiang Meng, Ziang Ma, Jie Ma, Jianbing Zhang, Rui |
| Editor | Papalia, Rocco |
| Copyright Year | 2023 |
| Abstract | Osteoarthritis (OA), the most common type of arthritis, is an age-associated disease, characterized by the progressive degradation of articular cartilage, synovial inflammation, and degeneration of subchondral bone. Chondrocyte proliferation is regulated by the Indian hedgehog (IHH in humans, Ihh in animals) signaling molecule, which regulates hypertrophy and endochondral ossification in the development of the skeletal system. microRNAs (miRNAs, miRs) are a family of about 22-nucleotide endogenous non-coding RNAs, which negatively regulate gene expression. In this study, the expression level of IHH was upregulated in the damaged articular cartilage tissues among OA patients and OA cell cultures, while that of miR-199a-5p was the opposite. Further investigations demonstrated that miR-199a-5p could directly regulate IHH expression and reduce chondrocyte hypertrophy and matrix degradation via the IHH signal pathway in the primary human chondrocytes. The intra-articular injection of synthetic miR-199a-5p agomir attenuated OA symptoms in rats, including the alleviation of articular cartilage destruction, subchondral bone degradation, and synovial inflammation. The miR-199a-5p agomir could also inhibit the Ihh signaling pathway in vivo. This study might help in understanding the role of miR-199a-5p in the pathophysiology and molecular mechanisms of OA and indicate a potential novel therapeutic strategy for OA patients. |
| Journal | Journal of Clinical Medicine [J Clin Med] |
| Volume Number | 12 |
| DOI | 10.3390/jcm12041313 |
| PubMed Central reference number | PMC9959662 |
| Issue Number | 4 |
| PubMed reference number | 36835852 |
| e-ISSN | 20770383 |
| Language | English |
| Publisher | Molecular Diversity Preservation International (MDPI) |
| Publisher Date | 2023-02-07 |
| Access Restriction | Open |
| Subject Keyword | miR-199a-5p osteoarthritis cartilage chondrocyte intra-articular injection |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine |
