Safety and efficacy of a feed additive consisting of endo-1,4-β-d-mannanase produced by Paenibacillus lentus DSM 33618 (Hemicell® HT/HT-L) for chickens and turkeys for fattening, chickens reared for laying, turkeys reared for breeding, minor poultry species to point of lay, pigs for fattening, weaned piglets and minor porcine species (Elanco GmbH).

Content Provider	Europe PMC
Author	Bampidis, Vasileios Azimonti, Giovanna de Lourdes Bastos, Maria Christensen, Henrik Durjava, Mojca Dusemund, Birgit Kouba, Maryline López‐Alonso, Marta López Puente, Secundino Marcon, Francesca Mayo, Baltasar Pechová, Alena Petkova, Mariana Ramos, Fernando Sanz, Yolanda Villa, Roberto Edoardo Woutersen, Ruud Anguita, Montserrat Brozzi, Rosella García‐Cazorla, Yolanda Ortuño, Jordi Pettenati, Elisa Vettori, Maria Vittoria Revez, Joana
Abstract	AbstractFollowing a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of endo‐1,4‐β‐d‐mannanase (Hemicell® HT/HT‐L) produced by a genetically‐modified strain of Paenibacillus lentus (DSM 33618) as a zootechnical feed additive for chickens and turkeys for fattening, chickens reared for laying, turkeys reared for breeding, minor poultry species to point of lay, pigs for fattening, piglets (weaned) and minor porcine species. The production strain was obtained from a Paenibacillus lentus recipient strain that has been evaluated previously by EFSA and considered to be safe. The genetic modification does not raise safety concerns and there were no antibiotic resistance genes from the genetic modification in the production strain. Viable cells and the DNA of the production strain were not found in the intermediate product used to formulate the additive. Hemicell® HT/HT‐L produced by Paenibacillus lentus DSM 33618 is considered safe for the above‐mentioned target species at the intended conditions of use. The use of Hemicell® HT/HT‐L as a feed additive raises no concerns for the consumer or for the environment. Hemicell® HT/HT‐L is not irritant to the skin and eyes but is regarded as a dermal sensitiser and a potential respiratory sensitiser. The additive has a potential to be efficacious at 32,000 U/kg in chickens for fattening, chickens reared for laying, minor poultry species for fattening or reared for laying/breeding, pigs for fattening and minor porcine species, and at 48,000 U/kg in turkeys for fattening, turkeys reared for breeding and weaned piglets.
Page Count	12
Volume Number	21
PubMed Central reference number	PMC9951083
Issue Number	2
PubMed reference number	36846382
Journal	EFSA Journal [EFSA J]
e-ISSN	18314732
DOI	10.2903/j.efsa.2023.7878
Language	English
Publisher	John Wiley and Sons Inc.
Publisher Date	2023-02-24
Publisher Place	Hoboken
Access Restriction	Open
Rights License	This is an open access article under the terms of the http://creativecommons.org/licenses/by-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited and no modifications or adaptations are made. © 2023 European Food Safety Authority. EFSA Journal published by Wiley‐VCH GmbH on behalf of European Food Safety Authority.
Subject Keyword	zootechnical additives digestibility enhancers Hemicell® HT/HT‐L endo‐1,4‐β‐d‐mannanase Paenibacillus lentus DSM 33618 safety efficacy
Content Type	Text
Resource Type	Article
Subject	Microbiology Food Science Plant Science Parasitology Animal Science and Zoology Veterinary