Phytochemical Screening and Antimicrobial Activity Evaluation of Selected Medicinal Plants in Ethiopia.

Content Provider	Europe PMC
Author	Dubale, Sileshi Kebebe, Dereje Zeynudin, Ahmed Abdissa, Negera Suleman, Sultan
Copyright Year	2023
Abstract	BackgroundThe emergence and spread of resistant microbes continue to be a major public health concern. Effective treatment alternatives, particularly from traditionally used medicinal plants, are needed.ObjectiveThe main objective of this study was to conduct phytochemical screening and antimicrobial activity evaluation of selected traditionally used medicinal plants in Ethiopia.MethodsThe ethnomedicinal use value frequency index (FI) was used to select twelve medicinal plants. Phytochemical classes of compounds were screened using different standard methods. Anti-microbial activities of plant extracts were evaluated against Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, and Candida albicans. Minimum inhibitory concentrations were measured using the broth micro-dilution method. The data were analyzed using Statistical Package for the Social Sciences (SPSS) version 21.0 and the findings were presented descriptively and using non parametric one-way ANOVA analysis (Kruskal–Wallis/Ddunn’s test).ResultsThe phytochemical constituents identified were flavonoids, alkaloids, glycosides, phenols, saponins, steroids, and terpenoids, with flavonoids, alkaloids, and phenols being the most abundant. The crude extracts and chloroform fractions of the extracts showed an activity against the tested strains. The crude extract of Thalictrum rhynchocarpum Quart.-Dill. and A.Rich root demonstrated superior activity against all the tested strains with the lowest minimum inhibitory concentrations of 0.48 μg/mL against Staphylococcus aureus and Escherichia coli; 0.98 μg/mL against Klebsiella pneumoniae, Pseudomonas aeruginosa; and 3.90 μg/mL against Candida albicans, which are even better than the reference drug, gentamicin and clotrimazole.ConclusionThe majority of evaluated medicinal plants demonstrated remarkable activity against tested microbial strains, which can be attributed to the presence of secondary metabolites of different classes of compounds. The finding provided scientific evidence for the use of these traditionally used medicinal plants.
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Page Count	12
Journal	Journal of Experimental Pharmacology [J Exp Pharmacol]
Volume Number	15
PubMed Central reference number	PMC9922502
PubMed reference number	36789235
e-ISSN	11791454
DOI	10.2147/jep.s379805
Language	English
Publisher	Dove
Publisher Date	2023-02-08
Access Restriction	Open
Rights License	This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). © 2023 Dubale et al.
Subject Keyword	traditional medicine medicinal plants phytochemical screening antimicrobial activity minimum inhibitory concentration
Content Type	Text
Resource Type	Article
Subject	Pharmacology (medical) Molecular Medicine Pharmacology