β-Lactamase Producing Escherichia coli Encoding bla_CTX-M and bla_CMY Genes in Chicken Carcasses from Egypt.

Content Provider	Europe PMC
Author	Abo-Almagd, Elham Elsayed Sabala, Rana Fahmi Abd-Elghany, Samir Mohammed Jackson, Charlene R. Ramadan, Hazem Imre, Kálmán Morar, Adriana Herman, Viorel Sallam, Khalid Ibrahim
Editor	Lavilla, Maria Amárita, Félix
Copyright Year	2023
Abstract	Escherichia coli with multidrug resistance and β-lactamase genes may constitute a great public health hazard due to the potential for their transmission to humans through the food chain. This study determined the prevalence, antibiotic resistance profiles, phylogroups, and β-lactamase genes of E. coli isolates from chicken carcasses marketed in Mansoura, Egypt. Interestingly, E. coli was detected in 98% (98/100) of the chicken carcasses examined, which seemed among the highest contamination rates by E. coli worldwide. From the 425 genetically verified uidA gene-positive E. coli, 85 isolates were further studied for antimicrobial resistance profiles, phylogroups, and β-lactamase genes. Interestingly, 89.41% of E. coli (76/85) strains tested against 24 different antibiotics were multidrug-resistant. Of the examined 85 E. coli isolates, 22 (25.88%) isolates harbored blaCTX-M and were resistant to ampicillin, cefazoline, and ceftriaxone, while three of them were resistant to ceftazidime besides. Nine (10.59%) E. coli strains harbored AmpC- β-lactamase blaCMY and were resistant to ampicillin. One isolate co-carried blaCMY and blaCTX-M genes, though it was negative for the blaTEM gene. Of the 35 isolates that harbored either extended-spectrum β-lactamase (ESBL) and/or AmpC β-lactamase genes, six strains (17.14%) were assigned to pathogenic phylogroup F and one to phylogroup E, whereas 28 (80%) isolates belonged to commensal phylogenetic groups.
Journal	Foods (Basel, Switzerland)
Volume Number	12
PubMed Central reference number	PMC9914308
Issue Number	3
PubMed reference number	36766128
e-ISSN	23048158
DOI	10.3390/foods12030598
Language	English
Publisher	MDPI
Publisher Date	2023-02-01
Access Restriction	Open
Rights License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors.
Subject Keyword	E. coli multidrug resistance β-lactamase phylogroups chicken carcasses
Content Type	Text
Resource Type	Article
Subject	Microbiology Health (social science) Health Professions Food Science Plant Science