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Laccase-Carrying Polylactic Acid Electrospun Fibers, Advantages and Limitations in Bio-Oxidation of Amines and Alcohols.
| Content Provider | Europe PMC |
|---|---|
| Author | Giraldi, Valentina Focarete, Maria Letizia Giacomini, Daria |
| Editor | Yu, Changmin |
| Copyright Year | 2022 |
| Abstract | Laccases are oxidative enzymes that could be good candidates for the functionalization of biopolymers with several applications as biosensors for the determination of bioactive amine and alcohols, for bioremediation of industrial wastewater, and for greener catalysts in oxidation reactions in organic synthesis, especially used for non-phenolic compounds in combination with redox mediators in the so-called Laccase Mediator System (LMS). In this work, we describe the immobilization of Laccase from Trametes versicolor (LTv) in poly-L-lactic acid (PLLA) nanofibers and its application in LMS oxidation reactions. The PLLA-LTv catalysts were successfully produced by electrospinning of a water-in-oil emulsion with an optimized method. Different enzyme loadings (1.6, 3.2, and 5.1% w/w) were explored, and the obtained mats were thoroughly characterized. The actual amount of the enzyme in the fibers and the eventual enzyme leaching in different solvents were evaluated. Finally, the PLLA-LTv mats were successfully applied as such in the oxidation reaction of catechol, and in the LMS method with TEMPO as mediator in the oxidation of amines with the advantage of easier work-up procedures by the immobilized enzyme. However, the PLLA-LTv failed the oxidation of alcohols with respect to the free enzyme. A tentative explanation was provided. |
| Journal | Journal of Functional Biomaterials |
| Volume Number | 14 |
| PubMed Central reference number | PMC9866953 |
| Issue Number | 1 |
| PubMed reference number | 36662071 |
| e-ISSN | 20794983 |
| DOI | 10.3390/jfb14010025 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-12-31 |
| Access Restriction | Open |
| Rights License | Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2022 by the authors. |
| Subject Keyword | enzyme catalysis laccases electrospinning polylactic acid oxidation amines |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biomaterials Biomedical Engineering |
