Volatiles from Pseudomonas palleroniana Strain B-BH16-1 Suppress Aflatoxin Production and Growth of Aspergillus flavus on Coix lacryma-jobi during Storage.

Content Provider	Europe PMC
Author	Zhou, Shihua Yuan, Qing-Song Wang, Xiaoai Jiang, Weike Ou, Xiaohong Yang, Changgui Gao, Yanping Wang, Yanhong Guo, Lanping Huang, Luqi Zhou, Tao
Copyright Year	2023
Abstract	Semen coicis is not only a traditional Chinese medicine (TCM), but also a typical food in China, with significant medical and healthcare value. Because semen coicis is rich in starch and oil, it can be easily contaminated with Aspergillus flavus and its aflatoxins (AFs). Preventing and controlling the contamination of semen coicis with Aspergillus flavus and its aflatoxins is vital to ensuring its safety as a drug and as a food. In this study, the endosphere bacteria Pseudomonas palleroniana strain B-BH16-1 produced volatiles that strongly inhibited the mycelial growth and spore formation activity of A. flavus. Gas chromatography–mass spectrometry profiling revealed three volatiles emitted from B-BH16-1, of which 1-undecene was the most abundant. We obtained authentic reference standards for these three volatiles; these significantly reduced mycelial growth and sporulation in Aspergillus, with dimethyl disulfide showing the most robust inhibitory activity. Strain B-BH16-1 was able to completely inhibit the biosynthesis of aflatoxins in semen coicis samples during storage by emitting volatile bioactive components. The microscope revealed severely damaged mycelia and a complete lack of sporulation. This newly identified plant endophyte bacterium was able to strongly inhibit the sporulation and growth of Aspergillus and the synthesis of associated mycotoxins, thus not only providing valuable information regarding an efficient potential strategy for the prevention of A. flavus contamination in TCM and food, but potentially also serving as a reference in the control of toxic fungi.
Journal	Toxins (Basel)
Volume Number	15
PubMed Central reference number	PMC9861347
Issue Number	1
PubMed reference number	36668896
e-ISSN	20726651
DOI	10.3390/toxins15010077
Language	English
Publisher	MDPI
Publisher Date	2023-01-14
Access Restriction	Open
Rights License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors.
Subject Keyword	semen coicis Aspergillus flavus aflatoxins Pseudomonas palleroniana volatiles endosphere bacteria
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Toxicology