Single-Molecule Peptide Identification Using Fluorescence Blinking Fingerprints.

Content Provider	Europe PMC
Author	Püntener, Salome Rivera-Fuentes, Pablo
Copyright Year	2023
Abstract	The ability to identifypeptides with single-molecule sensitivitywould lead to next-generation proteomics methods for basic researchand clinical applications. Existing single-molecule peptide sequencingmethods can read some amino acid sequences, but they are limited intheir ability to distinguish between similar amino acids or post-translationalmodifications. Here, we demonstrate that the fluorescence intermittencyof a peptide labeled with a spontaneously blinking fluorophore containsinformation about the structure of the peptide. Using a deep learningalgorithm, this single-molecule blinking pattern can be used to identifythe peptide. This method can distinguish between peptides with differentsequences, peptides with the same sequence but different phosphorylationpatterns, and even peptides that differ only by the presence of epimerizedresidues. This study builds the foundation for a targeted proteomicsmethod with single-molecule sensitivity.
ISSN	00027863
Volume Number	145
PubMed Central reference number	PMC9853850
Issue Number	2
PubMed reference number	36603184
Journal	Journal of the American Chemical Society [J. Am. Chem. Soc]
e-ISSN	15205126
DOI	10.1021/jacs.2c12561
Language	English
Publisher	American Chemical Society
Publisher Date	2023-01-05
Access Restriction	Open
Rights License	Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). © 2023 The Authors. Published by American Chemical Society
Content Type	Text
Resource Type	Article
Subject	Chemistry Colloid and Surface Chemistry Biochemistry Catalysis