Graphitic Carbon Nitride-Wrapped Metal-free PoPD-Based Biosensor for Xanthine Detection.

Content Provider	Europe PMC
Author	Thakur, Deeksha Pandey, Chandra Mouli Kumar, Devendra
Copyright Year	2022
Abstract	A metal-free, enzymatic biosensor was developed usinggraphiticcarbon nitride (g-C3N4)-wrapped poly-ortho-phenylenediamine (PoPD) for the determinationof xanthine (Xn). Field emission scanning electron microscopy, Fouriertransform infrared spectroscopy, and X-ray diffraction confirmed thesuccessful formation of the PoPD, g-C3N4 nanosheets and PoPD@g-C3N4 nanocomposite.Furthermore, the electrochemical behavior of the biosensor was characterizedby cyclic voltammetry and electrochemical impedance spectroscopy.The prepared enzyme electrode exhibited maximum response at pH 7.5with a response time of 5 s, and its sensitivity was 5.798 μAM–1. The nanocomposite shows exceptional sensing capabilitiesfor detecting Xn, having a wide linear range from 1 nM to 1 μMwith a relatively low detection limit of 0.001 nM. The biosensor showsgood stability (4 weeks) and reproducibility and can detect the presenceof Xn from other interfering analytes. Validation of the biosensorwith real samples obtained from Rohu (Labeo rohita) fish shows that the fabricated biosensor has the requisite potentialto be used for Xn detection in meat samples.
Journal	ACS Omega
Volume Number	8
PubMed Central reference number	PMC9851023
Issue Number	2
PubMed reference number	36687095
e-ISSN	24701343
DOI	10.1021/acsomega.2c06727
Language	English
Publisher	American Chemical Society
Publisher Date	2022-12-30
Access Restriction	Open
Rights License	Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). © 2022 The Authors. Published by American Chemical Society
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering