Real-time six-dimensional spatiotemporal tracking of single anisotropic nanoparticles in live cells by integrated multifunctional light-sheet nanoscopy.

Content Provider	Europe PMC
Author	Cao, Yingying Lee, Seungah Kim, Kyungsoo Kwak, Jong-Young Kang, Seong Ho
Abstract	An integrated multifunctional light-sheet nanoscopy (iMLSN) combined with differential interference contrast, total internal reflection, epifluorescence, a super-resolution radial fluctuation-stream module, and a wavelength-dependent light sheet was developed to simultaneously realize the six-dimensional (6D) vector-valued (three coordinates + rotational dynamics (azimuth and elevation angles) + transport speed) tracking of anisotropic nanoparticles in single living cells. The wavelength-dependent asymmetric scattering of light by gold nanorods was used to trigger signals depending on the polarizer angle, and real-time photo-switching was achieved by turning the polarizer, obtaining a series of super-resolution images, and tracking using different polarization directions and two channels. This technique was employed to directly observe native gold nanorods (AuNRs; 5 nm diameter × 15 nm length) and surface-functionalized AuNRs during their endocytosis and transport at the upper and attaching side membrane regions of single living cells, revealing that the AuNRs bound to the membrane receptors. The nanorods were subsequently internalized and transported away from the original entry spots. Detailed dynamic information regarding the rotation properties and endocytosis speed during the transmembrane process was also acquired for each region. The developed technique can be considered useful for the real-time monitoring of intracellular transport at various regions in single living cells, as well as for 6D vector-valued non-fluorescence super-resolution imaging and tracking.Graphical AbstractSupplementary InformationThe online version contains supplementary material available at 10.1007/s00604-023-05633-1.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC9841004&blobtype=pdf
ISSN	00263672
Journal	Mikrochimica Acta [Mikrochim Acta]
Volume Number	190
DOI	10.1007/s00604-023-05633-1
PubMed Central reference number	PMC9841004
Issue Number	2
PubMed reference number	36642770
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2023-01-16
Publisher Place	Vienna
Access Restriction	Open
Rights License	Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2023
Subject Keyword	Six-dimensional tracking Anisotropic nanoparticle Multifunctional light-sheet nanoscopy Single living cell Bioimaging Fucoidan
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry