Antibacterial Thiopeptide GE2270-Congeners from Nonomuraea jiangxiensis.

Content Provider	Europe PMC
Author	Ching, Kuan-Chieh Chin, Elaine J. Wibowo, Mario Tan, Zann Y. Yang, Lay-Kien Seow, Deborah C. Leong, Chung-Yan Ng, Veronica W. Ng, Siew-Bee Kanagasundaram, Yoganathan
Editor	Majtan, Juraj
Copyright Year	2022
Abstract	Thiopeptides are macrocyclic natural products with potent bioactivity. Nine new natural thiopeptides (1−9) were obtained from a Nonomuraea jiangxiensis isolated from a terrestrial soil sample collected in Singapore. Even though some of these compounds were previously synthesized or isolated from engineered strains, herein we report the unprecedented isolation of these thiopeptides from a native Nonomuraea jiangxiensis. A comparison with the literature and a detailed analysis of the NMR and HRMS of compounds 1−9 was conducted to assign their chemical structures. The structures of all new compounds were highly related to the thiopeptide antibiotics GE2270, with variations in the substituents on the thiazole and amino acid moieties. Thiopeptides 1−9 exhibited a potent antimicrobial activity against the Gram-positive bacteria, Staphylococcus aureus with MIC90 values ranging from 2 µM to 11 µM. In addition, all compounds were investigated for their cytotoxicity against the human cancer cell line A549, none of the compounds were cytotoxic.
Journal	Molecules
Volume Number	28
DOI	10.3390/molecules28010101
PubMed Central reference number	PMC9822014
Issue Number	1
PubMed reference number	36615295
e-ISSN	14203049
Language	English
Publisher	Molecular Diversity Preservation International (MDPI)
Publisher Date	2022-12-23
Access Restriction	Open
Subject Keyword	thiopeptide GE2270 antibacterial natural products RiPPs structure elucidation
Content Type	Text
Resource Type	Article
Subject	Physical and Theoretical Chemistry Medicine Chemistry Drug Discovery Pharmaceutical Science Analytical Chemistry Molecular Medicine Organic Chemistry