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VANL-100 Attenuates Beta-Amyloid-Induced Toxicity in SH-SY5Y Cells.
| Content Provider | Europe PMC |
|---|---|
| Author | Collins, Andrila E. Saleh, Tarek M. Kalisch, Bettina E. |
| Editor | Tenore, Gian Carlo |
| Copyright Year | 2022 |
| Abstract | Antioxidants are being explored as novel therapeutics for the treatment of neurodegenerative diseases such as Alzheimer's disease (AD) through strategies such as chemically linking antioxidants to synthesize novel co-drugs. The main objective of this study was to assess the cytoprotective effects of the novel antioxidant compound VANL-100 in a cellular model of beta-amyloid (Aβ)-induced toxicity. The cytotoxic effects of Aβ in the presence and absence of all antioxidant compounds were measured using the 3-(4,5-dimethylthiazol-2-yl)2-5-diphenyl-2H-tetrazolium bromide (MTT) assay in SH-SY5Y cells in both pre-treatment and co-treatment experiments. In pre-treatment experiments, VANL-100, or one of its parent compounds, naringenin (NAR), alpha-lipoic acid (ALA), or naringenin + alpha-lipoic acid (NAR + ALA), was administrated 24 h prior to an additional 24-h incubation with 20 μM non-fibril or fibril Aβ25-35. Co-treatment experiments consisted of simultaneous treatment with Aβ and antioxidants. Pre-treatment and co-treatment with VANL-100 significantly attenuated Aβ-induced cell death. There were no significant differences between the protective effects of VANL-100, NAR, ALA, and NAR + ALA with either form of Aβ, or in the effect of VANL-100 between 24-h pre-treatment and co-treatment. These results demonstrate that the novel co-drug VANL-100 is capable of eliciting cytoprotective effects against Aβ-induced toxicity. |
| Journal | International Journal of Molecular Sciences [Int J Mol Sci] |
| Volume Number | 24 |
| DOI | 10.3390/ijms24010442 |
| PubMed Central reference number | PMC9820495 |
| Issue Number | 1 |
| PubMed reference number | 36613883 |
| e-ISSN | 14220067 |
| Language | English |
| Publisher | Molecular Diversity Preservation International (MDPI) |
| Publisher Date | 2022-12-27 |
| Access Restriction | Open |
| Subject Keyword | Alzheimer’s disease beta-amyloid antioxidants naringenin alpha lipoic acid |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physical and Theoretical Chemistry Molecular Biology Spectroscopy Catalysis Computer Science Applications Medicine Inorganic Chemistry Organic Chemistry |
