Cytotoxicity effects of nanohybrid, bulk-fill, and ormocer composites on dental pulp stem cells and human gingival fibroblast cells.

Content Provider	Europe PMC
Author	Hoseinifar, Razieh Eskandarizadeh, Ali Lashkarizadeh, Lida Derakhshani, Ali Arjmand, Fatemeh
Copyright Year	2022
Abstract	Background:Despite significant improvements in the physical and esthetic properties of modern composite resins, there are still concerns about their biocompatibility. The aim of the current study was to evaluate the toxicity of X-tra fil, Grandio, and Admira Fusion composites on dental pulp stem cells (DPSCs) and human gingival fibroblast (HGF) cells.Materials and Methods:In this in vitro experimental study, 48 composite disks were made using Grandio, Admira Fusion (2 mm high and 4 mm in diameter), and X-tra fil (4 mm high and 4 mm in diameter) composites and cured for 40 s. The composite blocks were then crushed with a sterile mortar and dissolved in phosphate saline buffer solution. Tetrazolium salt (3-(4,5-dimethyl thiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT_, neutral red (NR) assay, flow cytometry, and quantitative real-time polymerase chain reaction (RT-PCR) tests (n = 5) were used to evaluate the toxicity of the composites on two cell types (HGF, DPSCs). Data were analyzed using one-way ANOVA test followed by Newman–Keuls test. Level of significance was set at P < 0.05.Results:According to the results of MTT test, only Grandio showed a significant cytotoxicity in DPSCs, but in HGF cells, Grandio and X-tra fil both showed a significant cytotoxicity. In NR test, Grandio and X-tra fil composites showed a significant cytotoxicity on both HGF and DPSC cells. RT-PCR test results on both DPSC and HGF cells indicated that bax gene expression in the Grandio composite was significant. In this test, the nonexpression of the bcl2 gene in DPSCs was significant in Grandio (100 and 200 μg/ml) and in X-tra fil (200 μg/ml). All of the tests performed in this study showed no significant toxicity of Admira fusion.Conclusion:Admira Fusion is suitable for oral cells in terms of biocompatibility and can be used as a suitable restorative material for deep restorations near the pulp or adjacent to the gums.
ISSN	17353327
Journal	Dental Research Journal
Volume Number	19
PubMed Central reference number	PMC9808008
PubMed reference number	36605150
e-ISSN	20080255
Language	English
Publisher	Wolters Kluwer - Medknow
Publisher Date	2022-11-17
Publisher Place	India
Access Restriction	Open
Rights License	This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. Copyright: © 2022 Dental Research Journal
Subject Keyword	Cells composite resins dental pulp fibroblasts
Content Type	Text
Resource Type	Article
Subject	Dentistry