Fabrication of Curcumin Diethyl γ-Aminobutyrate-Loaded Chitosan-Coated Magnetic Nanocarriers for Improvement of Cytotoxicity against Breast Cancer Cells.

Content Provider	Europe PMC
Author	Hansapaiboon, Supakarn Bulatao, Bryan Paul Sorasitthiyanukarn, Feuangthit Niyamissara Jantaratana, Pongsakorn Nalinratana, Nonthaneth Vajragupta, Opa Rojsitthisak, Pranee Rojsitthisak, Pornchai
Editor	Stan, Raluca Ghitman, Jana
Copyright Year	2022
Abstract	This study shows the effectiveness of magnetic-guide targeting in the delivery of curcumin diethyl γ-aminobutyrate (CUR-2GE), a prodrug of curcumin (CUR) previously synthesized to overcome unfavorable physicochemical properties of CUR. In this study, chitosan (Ch)-coated iron oxide nanoparticles (Ch-IONPs) were fabricated and optimized using Box–Behnken design-based response surface methodology for delivery of CUR-2GE. Ch was used as a coating material on the nanoparticle surface to avoid aggregation. The optimized condition for preparing Ch-IONPs consisted of using 4 mg Ch fabricated at pH 11 under a reaction temperature of 85 °C. The optimized Ch-IONPs were successfully loaded with CUR-2GE with sufficient loading capacity (1.72 ± 0.01%) and encapsulation efficiency (94.9 ± 0.8%). The obtained CUR-2GE-loaded Ch-IONPs (CUR-2GE-Ch-IONPs) exhibited desirable characteristics including a particle size of less than 50 nm based on TEM images, superparamagnetic property, highly crystalline IONP core, sufficient stability, and sustained-release profile. In the presence of permanent magnets, CUR-2GE-Ch-IONPs significantly increased cellular uptake and cytotoxicity toward MDA-MB-231 with a 12-fold increase in potency compared to free CUR-2GE, indicating the potential of magnetic-field assisted delivery of CUR-2GE-Ch-IONPs for the treatment of triple-negative breast cancer.
Journal	Polymers
Volume Number	14
PubMed Central reference number	PMC9785553
Issue Number	24
PubMed reference number	36559930
e-ISSN	20734360
DOI	10.3390/polym14245563
Language	English
Publisher	MDPI
Publisher Date	2022-12-19
Access Restriction	Open
Rights License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2022 by the authors.
Subject Keyword	iron oxide nanoparticles magnetic targeting delivery prodrug Box–Behnken design cytotoxicity
Content Type	Text
Resource Type	Article
Subject	Chemistry Polymers and Plastics