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Characterization of a Novel Alginate Lyase with Two Alginate Lyase Domains from the Marine Bacterium Vibrio sp. C42.
| Content Provider | Europe PMC |
|---|---|
| Author | Sun, Xiao-Meng Xue, Zhao Sun, Mei-Ling Zhang, Yi Zhang, Yu-Zhong Fu, Hui-Hui Zhang, Yu-Qiang Wang, Peng |
| Editor | Lee, Youngdeuk |
| Copyright Year | 2022 |
| Abstract | Alginate is abundant in the cell walls of brown algae. Alginate lyases can degrade alginate, and thus play an important role in the marine carbon cycle and industrial production. Currently, most reported alginate lyases contain only one functional alginate lyase domain. AlyC8 is a putative alginate lyase with two alginate lyase domains (CD1 and CD2) from the marine alginate-degrading strain Vibrio sp. C42. To characterize AlyC8 and its two catalytic domains, AlyC8 and its two catalytic domain-deleted mutants, AlyC8-CD1 and AlyC8-CD2, were expressed in Escherichia coli. All three proteins have noticeable activity toward sodium alginate and exhibit optimal activities at pH 8.0–9.0 and at 30–40 °C, demonstrating that both CD1 and CD2 are functional. However, CD1 and CD2 showed opposite substrate specificity. The differences in substrate specificity and degradation products of alginate between the mutants and AlyC8 demonstrate that CD1 and CD2 can act synergistically to enable AlyC8 to degrade various alginate substrates into smaller oligomeric products. Moreover, kinetic analysis indicated that AlyC8-CD1 plays a major role in the degradation of alginate by AlyC8. These results demonstrate that AlyC8 is a novel alginate lyase with two functional catalytic domains that are synergistic in alginate degradation, which is helpful for a better understanding of alginate lyases and alginate degradation. |
| Journal | Marine Drugs |
| Volume Number | 20 |
| PubMed Central reference number | PMC9781882 |
| Issue Number | 12 |
| PubMed reference number | 36547893 |
| e-ISSN | 16603397 |
| DOI | 10.3390/md20120746 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-11-26 |
| Access Restriction | Open |
| Rights License | Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2022 by the authors. |
| Subject Keyword | alginate lyase catalytic domain alginate degradation PL7 lyase Vibrio marine bacterium |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology, Toxicology and Pharmaceutics Drug Discovery Pharmaceutical Science |
