Genomic Pattern of GSTM1 and T1 Gene Null polymorphism of Head and Neck Cancer Patients in Eastern India.

Content Provider	Europe PMC
Author	Surit, Richa Kumar, Santosh Sinha, Dinesh Kumar Shekhar, Ravi
Abstract	Objective:Homozygous deletion i.e., null polymorphism of the Glutathione S transferases genes hinders detoxification reactions by altering the sensitization of glutathione s transferases enzymes. Hence, we analysed the association between the GSTM1 and GSTT1 gene polymorphisms and head and neck cancer (HNC). Methods:The study consists of 238 healthy controls and 160 diagnosed cases of HNC, who attended the Regional Cancer Centre, Indira Gandhi Institute of Medical Sciences (a tertiary care hospital). DNA was extracted from whole blood of patients and control using Qiagen DNA extraction kit. GSTM1 and GSTT1 gene polymorphisms were examined using PCR and agarose gel electrophoresis. Results:GSTM0 null polymorphism was 26.25% and 15.13% in cases and control respectively. GSTT0 null polymorphism was observed in 18.13% cases and 8.82% in control groups. The GSTM0 null polymorphism was present significantly in case group as compared to control group (OR = 1.997, p = 0.006). There was also significant association of GSTT0 null polymorphism with case group as compared to control group (OR = 2.288, p = 0.006). The combined genotypes were also analysed. GSTM0T1 genotype (n = 27) was found to be most common among HNC group followed next by GSTM0T0 double deletion (n =15). Conclusion:The result indicated that there was strong association of GSTM0 and GSTT0 null polymorphism in those patients. The combined genotypes i.e., GSTM0T1 and GSTM0T0 null polymorphism also showed significant association in HNC patients.
ISSN	15137368
Volume Number	23
PubMed Central reference number	PMC9741883
Issue Number	8
PubMed reference number	36037119
Journal	Asian Pacific Journal of Cancer Prevention : APJCP [Asian Pac. J. Cancer Prev]
e-ISSN	2476762X
DOI	10.31557/APJCP.2022.23.8.2655
Language	English
Publisher	West Asia Organization for Cancer Prevention
Publisher Date	2022-08-01
Publisher Place	Iran
Access Restriction	Open
Rights License	This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License. https://creativecommons.org/licenses/by-nc/4.0/
Subject Keyword	Glutathione-s-transferase M1 gene Glutathione-s-transferase T1 gene Null polymorphism
Content Type	Text
Resource Type	Article
Subject	Public Health, Environmental and Occupational Health Cancer Research Epidemiology Oncology