NDLI: Effects of human chorionic gonadotropin-producing peripheral blood mononuclear cells on the endometrial receptivity and implantation sites of the mouse uterus.

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Effects of human chorionic gonadotropin-producing peripheral blood mononuclear cells on the endometrial receptivity and implantation sites of the mouse uterus.

Content Provider	Europe PMC
Author	Rezaee, Delsuz Bandehpour, Mojgan Kazemi, Bahram Hosseini, Sara Dehghan, Zeinab Bastaminejad, Saiyad Salehi, Mohammad
Copyright Year	2022
Abstract	ObjectiveThis research investigated the effects of human chorionic gonadotropin (HCG)-producing peripheral blood mononuclear cells (PBMCs) on the implantation rate and embryo attachment in mice.MethodsIn this experimental study, a DNA fragment of the HCG gene was cloned into an expression vector, which was transfected into PBMCs. The concentration of the produced HCG was measured using enzyme-linked immunosorbent assay. Embryo attachment was investigated on the co-cultured endometrial cells and PBMCs in vitro. As an in vivo experiment, intrauterine administration of PBMCs was done in plaque-positive female mice. Studied mice were distributed into five groups: control, embryo implantation dysfunction (EID), EID with produced HCG, EID with PBMCs, and EID with HCG-producing PBMCs. Uterine horns were excised to characterize the number of implantation sites and pregnancy rate on day 7.5 post-coitum. During an implantation window, the mRNA expression of genes was evaluated using real-time polymerase chain reaction.ResultsDNA fragments were cloned between the BamHI and EcoRI sites in the vector. About 465 pg/mL of HCG was produced in the transfected PBMCs. The attachment rate, pregnancy rate, and the number of implantation sites were substantially higher in the HCG-producing PBMCs group than in the other groups. Significantly elevated expression of the target genes was observed in the EID with HCG-producing PBMCs group.ConclusionAlterations in gene expression following the intrauterine injection of HCG-producing PBMCs, could be considered a possible cause of increased embryo attachment rate, pregnancy rate, and the number of implantation sites.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC9732076&blobtype=pdf
ISSN	22338233
Journal	Clinical and Experimental Reproductive Medicine [Clin Exp Reprod Med]
Volume Number	49
DOI	10.5653/cerm.2022.05358
PubMed Central reference number	PMC9732076
Issue Number	4
PubMed reference number	36482499
e-ISSN	22338241
Language	English
Publisher	Korean Society for Reproductive Medicine
Publisher Date	2022-11-25
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2022. THE KOREAN SOCIETY FOR REPRODUCTIVE MEDICINE
Subject Keyword	Human chorionic gonadotropin Infertility Embryo implantation Peripheral blood mononuclear cell
Content Type	Text
Resource Type	Article
Subject	Reproductive Medicine

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