Integrated Exposomics/Metabolomics for Rapid Exposure and Effect Analyses.

Content Provider	Europe PMC
Author	Flasch, Mira Fitz, Veronika Rampler, Evelyn Ezekiel, Chibundu N. Koellensperger, Gunda Warth, Benedikt
Copyright Year	2022
Abstract	The totality of environmentalexposures and lifestyle factors,commonly referred to as the exposome, is poorly understood. Measuringthe myriad of chemicals that humans are exposed to is immensely challenging,and identifying disrupted metabolic pathways is even more complex.Here, we present a novel technological approach for the comprehensive,rapid, and integrated analysis of the endogenous human metabolomeand the chemical exposome. By combining reverse-phase and hydrophilicinteraction liquid chromatography (HILIC) and fast polarity-switching,molecules with highly diverse chemical structures can be analyzedin 15 min with a single analytical run as both column’s effluentsare combined before analysis. Standard reference materials and authenticstandards were evaluated to critically benchmark performance. Highlysensitive median limits of detection (LODs) with 0.04 μM for>140 quantitatively assessed endogenous metabolites and 0.08 ng/mLfor the >100 model xenobiotics and human estrogens in solvent wereobtained. In matrix, the median LOD values were higher with 0.7 ng/mL(urine) and 0.5 ng/mL (plasma) for exogenous chemicals. To prove thedual-column approach’s applicability, real-life urine samplesfrom sub-Saharan Africa (high-exposure scenario) and Europe (low-exposurescenario) were assessed in a targeted and nontargeted manner. Ourliquid chromatography high-resolution mass spectrometry (LC-HRMS)approach demonstrates the feasibility of quantitatively and simultaneouslyassessing the endogenous metabolome and the chemical exposome forthe high-throughput measurement of environmental drivers of diseases.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC9709941&blobtype=pdf
Journal	JACS Au
Volume Number	2
DOI	10.1021/jacsau.2c00433
PubMed Central reference number	PMC9709941
Issue Number	11
PubMed reference number	36465551
e-ISSN	26913704
Language	English
Publisher	American Chemical Society
Publisher Date	2022-11-07
Access Restriction	Open
Rights License	Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). © 2022 The Authors. Published by American Chemical Society
Subject Keyword	exposome metabolome human biomonitoring high-resolution mass spectrometry exposure biomarker
Content Type	Text
Resource Type	Article
Subject	Chemistry Physical and Theoretical Chemistry Analytical Chemistry Organic Chemistry