Development and Validation of an LC-MS/MS Method for Simultaneous Determination of Short Peptide-Based Drugs in Human Blood Plasma.

Content Provider	Europe PMC
Author	Fisher, Elizaveta N. Melnikov, Evgeny S. Gegeckori, Vladimir Potoldykova, Natalya V. Enikeev, Dmitry V. Pavlenko, Kirill A. Agatonovic-Kustrin, Snezana Morton, David W. Ramenskaya, Galina V.
Editor	Liu, Xingang Xiao, Zhiming
Copyright Year	2022
Abstract	A novel HPLC-ESI-MS/MS method for simultaneous gonadotropin-releasing hormone (GnRH) analogs and somatostatin analog quantitation was developed and validated. The developed method was successfully applied to pharmacokinetic studies. The sample preparation process included solid-phase extraction (SPE). Effective chromatographic separation of the analytes and internal standard (dalargin) was achieved with a C18 column, using a gradient elution with two mobile phases: 0.1% v/v formic acid (aqueous solution) and 0.1% v/v formic acid (acetonitrile solution). The linearity of the method was demonstrated within a concentration range of 0.5-20 ng/mL, with correlation coefficients between 0.998-0.999 for goserelin, buserelin, triptorelin, and octreotide, respectively. The relative standard deviation (RSD, %) values for method accuracy and precision did not exceed 20% at the lower level of quantitation (LLOQ) or 15% at other concentration levels.
Journal	Molecules
Volume Number	27
DOI	10.3390/molecules27227831
PubMed Central reference number	PMC9695356
Issue Number	22
PubMed reference number	36431933
e-ISSN	14203049
Language	English
Publisher	Molecular Diversity Preservation International (MDPI)
Publisher Date	2022-11-14
Access Restriction	Open
Subject Keyword	LC-ESI-MS/MS peptide drugs GnRH analogs somatostatin analogue solid-phase extraction
Content Type	Text
Resource Type	Article
Subject	Physical and Theoretical Chemistry Medicine Chemistry Drug Discovery Pharmaceutical Science Analytical Chemistry Molecular Medicine Organic Chemistry