Optimization of the Fermentative Production of Rhizomucor miehei Lipase in Aspergillus oryzae by Controlling Morphology.

Content Provider	Europe PMC
Author	Li, Chao Xu, Dou Xiong, Zhiyue Yang, Yiming Tian, Guiwei Wu, Xuezhi Wang, Yonghong Zhuang, Yingping Chu, Ju Tian, Xiwei
Editor	Koller, Martin Bracewell, Daniel G.
Copyright Year	2022
Abstract	Morphology plays an important role in the fermentation bioprocess of filamentous fungi. In this study, we investigated the controlling strategies of morphology that improved the efficiency of Rhizomucor miehei lipase (RML) production using a high-yield Aspergillus oryzae. First, the inoculated spore concentrations were optimized in seed culture, and the RML activity increased by 43.4% with the well-controlled mycelium pellets in both ideal sizes and concentrations. Then, the initial nitrogen source and agitation strategies were optimized to regulate the morphology of Aspergillus oryzae in a 5 L bioreactor, and the established stable fermentation system increased the RML activity to 232.0 U/mL, combined with an increase in total RML activity from 98,080 U to 487,179 U. Furthermore, the optimized fermentation strategy was verified by a high-yield Aspergillus oryzae and achieved an additional improvement of RML activity, up to 320.0 U/mL. Moreover, this optimized fermentation bioprocess was successfully scaled up to a 50 L bioreactor, and the RML activity reached 550.0 U/mL. This work has established a stable precision fermentation bioprocess for RML production by A. oryzae in bioreactors, and the controlling strategy developed in this study could potentially be extended to an industrial scale for RML production with high efficiency.
Journal	Bioengineering
Volume Number	9
PubMed Central reference number	PMC9687634
Issue Number	11
PubMed reference number	36354521
e-ISSN	23065354
DOI	10.3390/bioengineering9110610
Language	English
Publisher	MDPI
Publisher Date	2022-10-25
Access Restriction	Open
Rights License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2022 by the authors.
Subject Keyword	Aspergillus oryzae Rhizomucor miehei lipase morphology spore concentration precision fermentation
Content Type	Text
Resource Type	Article
Subject	Bioengineering