Antibacterial effect of nano-chlorhexidine on Enterococcus faecalis biofilm in root canal system: An in vitro study.

Content Provider	Europe PMC
Author	Hajihassani, Neda Alavi, Orkideh Karamshahi, Mohamadjavad Marashi, Seyed Mahmoud Amin Khademi, Abbasali Mohammadi, Navid
Copyright Year	2022
Abstract	Background:A new method to improve the properties of the materials is nano-encapsulation, which improves the biological properties, antibacterial activity along with reduction of toxicity. Due to the spread of nano-knowledge, the present study was performed to evaluate the antibacterial effect of nano-chlorhexidine (CHX) on Enterococcus faecalis biofilm in the root canal system.Materials and Methods:In this in vitro experimental study, 55 matured single-root mandibular premolars were decoronated and the canals were prepared by single length method up to #F3 ProTaper Universal system. Five teeth were selected as negative control. Then, the teeth were randomly divided into three experimental groups (n = 15) and a positive control group (n = 5). The experimental groups were irrigated with 2% nano- CHX gel, 2% CHX solution, and 5.25% sodium hypochlorite (NaOCl), respectively. Finally, the number of colonies was counted. Kruskal–Wallis test was used to compare the number of colonies among groups. The level of significance was set at P < 0.05.Results:The mean number of colonies in the groups of nano-CHX, NaOCl, CHX, and positive control were obtained as 17.73 ± 18.69, 35.53 ± 36.42, 38.8 ± 31.8, and 96.8 ± 22.52, respectively. There was a significant decrease in the number of colonies in all the experimental groups compared to the control group (P < 0.05). However, difference in the number of colonies among these three groups was not significant (P > 0.05).Conclusion:The use of nano-CHX in removing E. faecalis biofilm from root canal is as effective as the use of CHX and NaOCl.
ISSN	17353327
Journal	Dental Research Journal
Volume Number	19
PubMed Central reference number	PMC9667490
PubMed reference number	36407778
e-ISSN	20080255
Language	English
Publisher	Wolters Kluwer - Medknow
Publisher Date	2022-09-26
Publisher Place	India
Access Restriction	Open
Rights License	This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. Copyright: © 2022 Dental Research Journal
Subject Keyword	Chlorhexidine Enterococcus faecalis nanoparticles sodium hypochlorite
Content Type	Text
Resource Type	Article
Subject	Dentistry