NDLI: Erythrosin B as a New Photoswitchable Spin Label for Light-Induced Pulsed EPR Dipolar Spectroscopy.

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Erythrosin B as a New Photoswitchable Spin Label for Light-Induced Pulsed EPR Dipolar Spectroscopy.

Content Provider	Europe PMC
Author	Bertran, Arnau Morbiato, Laura Aquilia, Sara Gabbatore, Laura De Zotti, Marta Timmel, Christiane R. Di Valentin, Marilena Bowen, Alice M.
Editor	Krumkacheva, Olesya A. Fedin, Matvey
Copyright Year	2022
Abstract	We present a new photoswitchable spin label for light-induced pulsed electron paramagnetic resonance dipolar spectroscopy (LiPDS), the photoexcited triplet state of erythrosin B (EB), which is ideal for biological applications. With this label, we perform an in-depth study of the orientational effects in dipolar traces acquired using the refocused laser-induced magnetic dipole technique to obtain information on the distance and relative orientation between the EB and nitroxide labels in a rigid model peptide, in good agreement with density functional theory predictions. Additionally, we show that these orientational effects can be averaged to enable an orientation-independent analysis to determine the distance distribution. Furthermore, we demonstrate the feasibility of these experiments above liquid nitrogen temperatures, removing the need for expensive liquid helium or cryogen-free cryostats. The variety of choices in photoswitchable spin labels and the affordability of the experiments are critical for LiPDS to become a widespread methodology in structural biology.
Journal	Molecules
Volume Number	27
DOI	10.3390/molecules27217526
PubMed Central reference number	PMC9657417
Issue Number	21
PubMed reference number	36364348
e-ISSN	14203049
Language	English
Publisher	Molecular Diversity Preservation International (MDPI)
Publisher Date	2022-11-03
Access Restriction	Open
Subject Keyword	electron paramagnetic resonance (EPR) electron spin resonance (ESR) pulsed dipolar spectroscopy (PDS) laser-induced magnetic dipole spectroscopy (LaserIMD) distance measurements orientational effects spin labels photogenerated triplet state erythrosin B high temperature
Content Type	Text
Resource Type	Article
Subject	Physical and Theoretical Chemistry Medicine Chemistry Drug Discovery Pharmaceutical Science Analytical Chemistry Molecular Medicine Organic Chemistry

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