Comparison of Osteoblastic Differentiation of Human Periodontal Ligament Stem Cells through Application of Two β-tricalcium Phosphate Products: An in vitro Study.

Content Provider	Europe PMC
Author	Mirkhani, Seyed Mohammad Hossein Amini Sedeh, Shirin Esfahanian, Vahid
Abstract	Statement of the Problem: Osteoblastic differentiation of periodontal ligament stem cells (PLSCs) is essentialfor alveolar bone regeneration.Purpose: The purpose of this study was to compare the potential of twoβ-tricalcium phosphate (βTCP) products to induce osteoblastic differentiation of human PLSCs.Materials and Method: In this in vitro study, human PLSCs were cultured in mediums supplementedwith Guidor Easy-Graft [βTCP+polylactide-co-glycolide (PLCG)+n-methyl-2-pyrrolidone (NMP)][Sunstar Company, Swiss] or Sorbone [βTCP] [Meta Company, South Korea] as two alloplastsexperimental groups, mesenchymal cells differentiated into osteoblasts without alloplast aspositive control group, and mesenchymal cells without osteoblastic induction as negative controlgroup. Osteoblastic differentiation was evaluated using Alizarine Red staining andspectrophotometry to assay calcium deposits and real-time polymerase chain reaction toexamine expression of alkaline phosphatase (ALP) and osteopontin (OPN) antigens on day 21.Data were analyzed by using SPSS 22 software and one-way ANOVA and Bonferonitests (p< 0.05).Results: Spectrophotometry confirmed that calcium deposits were higher in GuidorEasy-Graft group compared to Sorbone group (p< 0.001) and higher in twoexperimental groups than controls (p< 0.05). According to real-timepolymerase chainreaction, level of ALP expression was higher in Sorbone than Guidor and the levels of Guidor,positive control and negative control were equal; OPN levels of the positive control were morethan the other groups. OPN levels of Sobone, Guidor and negative control were the same.Conclusion: These findings indicated that Guidor Easy-Graft and Sorbone enhanced differentiationof human PLSCs to osteoblasts, and could be employed as appropriate bone-graft materials.
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ISSN	23456485
Journal	Journal of Dentistry [J Dent (Shiraz)]
Volume Number	23
PubMed Central reference number	PMC9652058
Issue Number	1 Suppl
Issue Number	1 suppl
PubMed reference number	36380833
e-ISSN	23456418
DOI	10.30476/dentjods.2021.86700.1350
Language	English
Publisher	Shiraz University of Medical Sciences
Publisher Date	2022-06-01
Publisher Place	Iran
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 Unported License, ( http://creativecommons.org/licenses/by/4.0/ ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright: © Journal of Dentistry
Subject Keyword	Periodontal ligament Alkaline phosphatase Osteopontin Bone Regeneration
Content Type	Text
Resource Type	Article
Subject	Dentistry